Enzymatic modification of phosphatidylcholine with n-3 PUFA from silkworm oil fatty acids

α-Linolenic acid (ALA) containing phosphatidylcholine (PC) was prepared by an enzymatic method employing natural substrates comprising of egg and eri silkworm oil. Eri silkworm oil extracted from eri pupae was saponified to obtain the fatty acid mixture which was further subjected to urea complexati...

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Bibliographic Details
Published in:Grasas y Aceites
Main Authors: Shanker Kaki, Shiva, Ravinder, T., Ashwini, B., Rao, B. V.S.K., Prasad, R. B.N.
Format: Article in Journal/Newspaper
Language:English
Published: Consejo Superior de Investigaciones Científicas 2014
Subjects:
α-linolenic acid
Egg phosphatidylcholine
Lipase
Nutraceuticals
Silkworm oil
Aceite de gusano de seda
Ácido α-linolénico
Fosfatidilcolina de huevo
Lipasa
Nutracéuticos
Tuvo
Huevo
Gusano
Antarc*
Antarctica
Online Access:https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1487
https://doi.org/10.3989/gya.097213
Description
Summary:α-Linolenic acid (ALA) containing phosphatidylcholine (PC) was prepared by an enzymatic method employing natural substrates comprising of egg and eri silkworm oil. Eri silkworm oil extracted from eri pupae was saponified to obtain the fatty acid mixture which was further subjected to urea complexation to obtain an ALA rich fraction with a purity of about 93%. Transesterification of PC with the ALA rich fraction with three immobilized lipases namely Lipozyme TL IM, Lipozyme RM IM and lipase from Candida Antarcticaz showed that only the lipase from Candida antarctica was successful for the incorporation of ALA into egg yolk PC. It was found that ALA was incorporated by up to 27% in the sn-1 position of egg PC and the positional distribution analysis of fatty acids in the modified PC showed that the sn-1 position was found to contain about 59% ALA. El ácido α-linolénico (ALA) contenido en fosfatidilcolina (PC) se preparó mediante un método enzimático empleando sustratos naturales que comprenden huevo y aceite de gusanos de seda. El aceite extraído de las crisálidas de gusanos de seda se saponificó para obtener la mezcla de ácidos grasos que se sometió a complejación con urea para obtener la fracción rica en ALA, con una pureza aproximadamente del 93%. La transesterificación de PC con fracción rica en ALA con tres lipasas inmovilizadas, Lipozyme TL IM, Lipozyme RM IM y lipasa de Candida antárctica, mostró que sólo la lipasa de Candida antarctica tuvo éxito en la incorporación de ALA en PC de yema de huevo. Se encontró que el ALA fue incorporado hasta 27% en la posición sn-1 de PC de huevo y el análisis de la distribución de los ácidos grasos en PC modificado mostró que la posición sn-1 que contenía aproximadamente 59% de ALA.

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