| Summary: | doktor The development of nanotechnology and the widespread use of silver nanoparticles in various industries is associated with a continuous quest for information on the potentially harmful effects of AgNPs on living organisms. Silver nanoparticles, due to their small size and unique physical and chemical properties, may exhibit genotoxic and cytotoxic effects against eukaryotic cells. When released into the environment, AgNPs remain in the environment, interacting with surrounding ecosystem components, posing a threat to human and animal health. To date, it has been shown that AgNPs can enter the body and subsequently deposit in tissues and organs. Within cells, silver nanoparticles can cause toxic effects by generating ROS, disturbed cell division or DNA damage. Due to different properties of AgNPs, which depend on the production method (chemical, physical, biological) it is extremely important to determine the effect of silver nanoparticles on the animal and human genome. Changes in nuclear chromatin induced by AgNPs can be observed at different stages of its formation, resulting in disruption of genome stability. Cytogenetic instability assays such as, comet assay, micronucleus assay or fragile site assay are used to determine the genotoxic and cytotoxic nature of AgNPs. The aim of this dissertation was to evaluate chromatin structure and nuclear DNA integrity in somatic cells of two selected representatives of the Canidae family: domestic dog (Canis familiaris) and blue fox (Alopex lagopus), treated with silver nanoparticles produced by the physical method of high voltage arc discharge, HVAD, in vitro using cytogenetic diagnostic tests: Fragile site, micronucleus variant CBMN and comet assay. An additional aim was to try to determine the genotoxic character of silver nanoparticles depending on the applied dose and time of cell exposure. The material for the study consisted of whole peripheral blood collected from two canine species: domestic dog and blue fox. For each species the experimental group ...
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