| Summary: | Given the known effect of lipid content on δ13C values and the potential effect of urea on δ15N values, examining the effects of lipid extraction, which can potentially extract both, is of particular importance for elasmobranch isotope ecology. Through analysing paired δ13C, total %C, δ15N, total %N and C:N values of non-lipid extracted (BULK) and lipid extracted (LE) muscle samples from twenty-one elasmobranch species, we assessed whether lipid extraction was required: (i) to remove lipids given reported low lipid content and, (ii) to determine if δ15N values were affected and whether this relates to the retention of isotopically light urea by elasmobranchs. The mean (± SD) δ13C values of eight out of twenty-one species significantly increased following lipid extraction with two species, the Greenland (Somniosus microcephalus) and whale (Rhincodon typus) shark, showing a marked increase (5.0 ± 0.4‰ and 3.3‰, respectively). The mean (± SD) and maximum increase in δ13C values were 0.6 ± 1.2‰ and 5.9‰, respectively. For δ15N data, thirteen species showed a significant increase following lipid extraction and a concomitant reduction in total percent nitrogen (%N). The C:N ratio for these species also increased from unexpectedly low values of < 3.0 to ~ 3.0, the value expected for pure protein. The mean and maximum observed increase in δ15N values were 0.6 ± 0.6‰ and 2.3‰, respectively. There was no effect of increasing animal size on δ13C and δ15N difference (LE–BULK) for the two species examined. Field sampled animals (sampled immediately upon capture in the marine environment) showed a greater δ15N difference than animals sampled in the laboratory (sampled several hours after capture in the marine environment) (1.0 ± 0.5‰ and 0.4 ± 0.4‰ respectively), while estuarine sampled animals (sampled immediately) showed the smallest difference (0.1 ± 0.6‰). The δ13C data demonstrate that lipid extraction is required to remove lipids from elasmobranch muscle tissue given both intra- and inter- species variability. In ...
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