| Summary: | The slow metabolism of bacteria at low temperatures affects the catalytic efficiency of enzymes and productivity. This article investigates the use of a psychrotolerant bacteria (Klebsiella sp. ABZ11) for biohydrogen production, yield and scaling at optimal temperature, pH and glucose in a batch fermentation process within a 2-liter bioreactor using Response Surface Methodology (RSM) to report the process performance. The results show 33.5°C, pH 6.75 and 9.15 g glucose as the optimal conditions. Scale-up yielded 137.56 mol/L biohydrogen, 22.13% more than production under optimized conditions. Biomass grew at 0.081/h and doubled in 17 h with 0.71 g cells to reach maximum production. Compared to 0.062/h, 22 h with 0.87 g cells in optimal condition to achieve maximum biohydrogen production. This result shows the potential of biohydrogen production using Antarctic psychrotolerant bacteria at mesophilic temperature.
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