Documenting the Southern Range Terminus of the Wood Frog (Lithobates sylvaticus) in North America

The Wood Frog (Lithobates sylvaticus) holds a remarkable position in North American amphibian biology, with its range extending from the Arctic Circle down to the near sub-tropical southeastern United States. This thesis presents a novel quantitative polymerase chain reaction analysis (qPCR) primer...

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Bibliographic Details
Main Author: Braswell, Christian
Format: Text
Language:unknown
Published: JSU Digital Commons 2024
Subjects:
Wood Frog (Lithobates sylvaticus)
Environmental DNA
Quantitative PCR
Range Terminus
PCR Primer
Appalachian Mountains
Biodiversity
Bioinformatics
Genetics
Molecular Genetics
Other Genetics and Genomics
Population Biology
Zoology
Arctic
Online Access:https://digitalcommons.jsu.edu/etds_theses/65
https://digitalcommons.jsu.edu/context/etds_theses/article/1066/viewcontent/auto_convert.pdf
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Summary:The Wood Frog (Lithobates sylvaticus) holds a remarkable position in North American amphibian biology, with its range extending from the Arctic Circle down to the near sub-tropical southeastern United States. This thesis presents a novel quantitative polymerase chain reaction analysis (qPCR) primer specific to L. sylvaticus and a survey effort regarding the southernmost distribution and detection of this species in Alabama through the application of environmental DNA (eDNA) sampling techniques. By investigating historical data and employing advanced genetic methodologies, this research provides insights into the contemporary status and distribution of the Wood Frog. This research is important to shed light on its adaptability to changing climates and habitats of a presumably thermosensitive species.

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