Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica

"Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants...

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Bibliographic Details
Published in:Process Biochemistry
Main Authors: García Echauri, Sergio Ángel, Ordoñez Acevedo, Leandro Gabriel, Rojas Contreras, Juan Antonio, Barba de la Rosa, Ana Paulina, De León Rodríguez, Antonio
Format: Article in Journal/Newspaper
Language:unknown
Published: Elsevier 2009
Subjects:
Antioxidant
Plant
Extremophil
Psychrophilic enzyme
Photo-oxidation
Oxidative stress
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
Antarc*
Antarctica
Online Access:http://hdl.handle.net/11627/3874
https://doi.org/10.1016/j.procbio.2009.04.021
Description
Summary:"Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 °C. However, zymogram test showed that the enzyme has more activity at 4 °C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods."

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