Ontogeny of bivalve immunity: assessing the potential of next-generation sequencing techniques

• Published in: Reviews in Aquaculture
• Main Authors: Bassim, Sleiman, Genard, Bertrand, Gauthier-Clerc, Sophie, Moraga, Dario, Tremblay, Rejean
• Other Authors: Institut des Sciences de la MER de Rimouski (ISMER), Université du Québec à Rimouski (UQAR), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Montréal (UdeM)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2015
• Subjects: ACL; bivalve; ontogeny; oyster crassostrea-gigas; transcriptomics; clams ruditapes-philippinarum; differential expression analysis; immune defence; massive mortality events; mussel mytilus-galloprovincialis; next-generation sequencing; peptidoglycan recognition protein; rna-seq data; scallop chlamys-farreri; toll-like receptors; vibrio-anguillarum challenge; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Crassostrea gigas
• Online Access: https://hal.science/hal-02554389; https://doi.org/10.1111/raq.12064

WOS:000362185000005 Living organisms are constantly evolving to secure their survival via adaptations at the molecular and cellular level. Most marine bivalves have microscopic planktonic larval stages until settlement to the benthic environment. These pelagic stages are generally more sensitive than their adult counterparts to environmental and pathogen threats. Adaptive capacities could improve survival of these early stages. Recent advancements in data mining and pipeline analysis should shed light on the currently unknown processes that occur during these first stages. Existing data on early stages are fragmented compared with the abundance of information available for adult. Exploring diversity through aquaculture and lessening the impact of common issues, for example, massive mortalities of larvae, especially within the current conditions of a changing climate, ultimately rests on our knowledge of the molecular processes responsible for phenotypic plasticity. Although it is somewhat difficult to assess immune mechanisms by tracking circulating immunocytes in larvae, studies on the development of immune processes are now feasible at the transcript level. Next-generation techniques offer outstanding solutions for wide-range transcriptome analysis. We present a short review of the early ontogeny of the immune system in marine bivalves, with particular focus on next-generation sequencing applications. Like all reviews of this nature, there is a trade-off between the depth of the coverage and the number of subjects discussed. We will thus restrict the scope to bivalve immunity and focus on the central concepts across a wide range of topics, that is, the ontogeny of immunity and advancements in molecular studies.