Novel insights into the genetic diversity of Parafavella based on mitochondrial CO1 sequences
International audience We used both nuclear ribosomal genes (28S rDNA, 18S rDNA, 5.8S rDNA, internal transcribed spacer regions: ITS1, ITS2) and mitochondrial CO1 sequences to group or distinguish morphotypes of Parafavella, a problematic genus of tintinnid ciliates of the marine microzooplankton. W...
Published in: | Zoologica Scripta |
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Online Access: | https://hal.sorbonne-universite.fr/hal-02337262 https://hal.sorbonne-universite.fr/hal-02337262/document https://hal.sorbonne-universite.fr/hal-02337262/file/Jung%20et%20al.%20-%202018%20-%20Novel%20insights%20into%20the%20genetic%20diversity%20of%20Paraf.pdf https://doi.org/10.1111/zsc.12312 |
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ftinsu:oai:HAL:hal-02337262v1 2023-11-05T03:40:54+01:00 Novel insights into the genetic diversity of Parafavella based on mitochondrial CO1 sequences Jung, Jae-Ho Moon, Ji Hye Park, Kyung-Min Kim, Sanghee Dolan, John Yang, Eun Jin Gangneung-Wonju National University Korea Polar Research Institute (KOPRI) Laboratoire d'océanographie de Villefranche (LOV) Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) 2018 https://hal.sorbonne-universite.fr/hal-02337262 https://hal.sorbonne-universite.fr/hal-02337262/document https://hal.sorbonne-universite.fr/hal-02337262/file/Jung%20et%20al.%20-%202018%20-%20Novel%20insights%20into%20the%20genetic%20diversity%20of%20Paraf.pdf https://doi.org/10.1111/zsc.12312 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/zsc.12312 hal-02337262 https://hal.sorbonne-universite.fr/hal-02337262 https://hal.sorbonne-universite.fr/hal-02337262/document https://hal.sorbonne-universite.fr/hal-02337262/file/Jung%20et%20al.%20-%202018%20-%20Novel%20insights%20into%20the%20genetic%20diversity%20of%20Paraf.pdf doi:10.1111/zsc.12312 info:eu-repo/semantics/OpenAccess ISSN: 0300-3256 EISSN: 1463-6409 Zoologica Scripta https://hal.sorbonne-universite.fr/hal-02337262 Zoologica Scripta, 2018, 47 (6), pp.743-755. ⟨10.1111/zsc.12312⟩ polymorphism marine plankton cryptic species Tintinnidea [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2018 ftinsu https://doi.org/10.1111/zsc.12312 2023-10-11T16:37:03Z International audience We used both nuclear ribosomal genes (28S rDNA, 18S rDNA, 5.8S rDNA, internal transcribed spacer regions: ITS1, ITS2) and mitochondrial CO1 sequences to group or distinguish morphotypes of Parafavella, a problematic genus of tintinnid ciliates of the marine microzooplankton. We sequenced 30 single cells of Parafavella from the Bering Sea, the Greenland Sea, and the East/Japan Sea. Sequences were obtained from 4 morphotypes, typically ascribed to P. gigantea, P. greenlandica, P. jorgenseni, and P. subrotundata and from GenBank, the nuclear ribosomal genes of P. parumdentata were retrieved. Cells of the five morphotypes had identical 18S and 5.8S gene sequences. ITS1, ITS2 and 28S sequences produced two clusters: one grouping P. greenlandica and P. parumdentata and the other grouping P. jorgenseni, P. gigantea, and P. subrotundata. In contrast, CO1 nucleotide data yielded eight haplotypes clustered into five groups: one composed of P. greenlandica morphotypes, two distinct haplotypes of P. jorgenseni, and two distinct haplotypes of P. gigantea with one including P. subrotundata morphotypes. We investigated the co‐occurrence of different morphotypes in samples from sites across a large gradient of latitude and concentrations of Parafavella cells. Natural communities contained 2–6 different morphotypes. We conclude that both crypticity and polymorphism characterize Parafavella, as known for other tintinnid genera. Article in Journal/Newspaper Bering Sea Greenland Greenland Sea Institut national des sciences de l'Univers: HAL-INSU Zoologica Scripta 47 6 743 755 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
polymorphism marine plankton cryptic species Tintinnidea [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
polymorphism marine plankton cryptic species Tintinnidea [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Jung, Jae-Ho Moon, Ji Hye Park, Kyung-Min Kim, Sanghee Dolan, John Yang, Eun Jin Novel insights into the genetic diversity of Parafavella based on mitochondrial CO1 sequences |
topic_facet |
polymorphism marine plankton cryptic species Tintinnidea [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
International audience We used both nuclear ribosomal genes (28S rDNA, 18S rDNA, 5.8S rDNA, internal transcribed spacer regions: ITS1, ITS2) and mitochondrial CO1 sequences to group or distinguish morphotypes of Parafavella, a problematic genus of tintinnid ciliates of the marine microzooplankton. We sequenced 30 single cells of Parafavella from the Bering Sea, the Greenland Sea, and the East/Japan Sea. Sequences were obtained from 4 morphotypes, typically ascribed to P. gigantea, P. greenlandica, P. jorgenseni, and P. subrotundata and from GenBank, the nuclear ribosomal genes of P. parumdentata were retrieved. Cells of the five morphotypes had identical 18S and 5.8S gene sequences. ITS1, ITS2 and 28S sequences produced two clusters: one grouping P. greenlandica and P. parumdentata and the other grouping P. jorgenseni, P. gigantea, and P. subrotundata. In contrast, CO1 nucleotide data yielded eight haplotypes clustered into five groups: one composed of P. greenlandica morphotypes, two distinct haplotypes of P. jorgenseni, and two distinct haplotypes of P. gigantea with one including P. subrotundata morphotypes. We investigated the co‐occurrence of different morphotypes in samples from sites across a large gradient of latitude and concentrations of Parafavella cells. Natural communities contained 2–6 different morphotypes. We conclude that both crypticity and polymorphism characterize Parafavella, as known for other tintinnid genera. |
author2 |
Gangneung-Wonju National University Korea Polar Research Institute (KOPRI) Laboratoire d'océanographie de Villefranche (LOV) Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Jung, Jae-Ho Moon, Ji Hye Park, Kyung-Min Kim, Sanghee Dolan, John Yang, Eun Jin |
author_facet |
Jung, Jae-Ho Moon, Ji Hye Park, Kyung-Min Kim, Sanghee Dolan, John Yang, Eun Jin |
author_sort |
Jung, Jae-Ho |
title |
Novel insights into the genetic diversity of Parafavella based on mitochondrial CO1 sequences |
title_short |
Novel insights into the genetic diversity of Parafavella based on mitochondrial CO1 sequences |
title_full |
Novel insights into the genetic diversity of Parafavella based on mitochondrial CO1 sequences |
title_fullStr |
Novel insights into the genetic diversity of Parafavella based on mitochondrial CO1 sequences |
title_full_unstemmed |
Novel insights into the genetic diversity of Parafavella based on mitochondrial CO1 sequences |
title_sort |
novel insights into the genetic diversity of parafavella based on mitochondrial co1 sequences |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.sorbonne-universite.fr/hal-02337262 https://hal.sorbonne-universite.fr/hal-02337262/document https://hal.sorbonne-universite.fr/hal-02337262/file/Jung%20et%20al.%20-%202018%20-%20Novel%20insights%20into%20the%20genetic%20diversity%20of%20Paraf.pdf https://doi.org/10.1111/zsc.12312 |
genre |
Bering Sea Greenland Greenland Sea |
genre_facet |
Bering Sea Greenland Greenland Sea |
op_source |
ISSN: 0300-3256 EISSN: 1463-6409 Zoologica Scripta https://hal.sorbonne-universite.fr/hal-02337262 Zoologica Scripta, 2018, 47 (6), pp.743-755. ⟨10.1111/zsc.12312⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/zsc.12312 hal-02337262 https://hal.sorbonne-universite.fr/hal-02337262 https://hal.sorbonne-universite.fr/hal-02337262/document https://hal.sorbonne-universite.fr/hal-02337262/file/Jung%20et%20al.%20-%202018%20-%20Novel%20insights%20into%20the%20genetic%20diversity%20of%20Paraf.pdf doi:10.1111/zsc.12312 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1111/zsc.12312 |
container_title |
Zoologica Scripta |
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47 |
container_issue |
6 |
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743 |
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1781697125674909696 |