Novel insights into the genetic diversity of Parafavella based on mitochondrial CO1 sequences

• Published in: Zoologica Scripta
• Main Authors: Jung, Jae-Ho, Moon, Ji Hye, Park, Kyung-Min, Kim, Sanghee, Dolan, John, Yang, Eun Jin
• Other Authors: Gangneung-Wonju National University, Korea Polar Research Institute (KOPRI), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2018
• Subjects: polymorphism; marine plankton; cryptic species; Tintinnidea; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology; Bering Sea; Greenland; Greenland Sea
• Online Access: https://hal.sorbonne-universite.fr/hal-02337262; https://hal.sorbonne-universite.fr/hal-02337262/document; https://hal.sorbonne-universite.fr/hal-02337262/file/Jung%20et%20al.%20-%202018%20-%20Novel%20insights%20into%20the%20genetic%20diversity%20of%20Paraf.pdf; https://doi.org/10.1111/zsc.12312

International audience We used both nuclear ribosomal genes (28S rDNA, 18S rDNA, 5.8S rDNA, internal transcribed spacer regions: ITS1, ITS2) and mitochondrial CO1 sequences to group or distinguish morphotypes of Parafavella, a problematic genus of tintinnid ciliates of the marine microzooplankton. We sequenced 30 single cells of Parafavella from the Bering Sea, the Greenland Sea, and the East/Japan Sea. Sequences were obtained from 4 morphotypes, typically ascribed to P. gigantea, P. greenlandica, P. jorgenseni, and P. subrotundata and from GenBank, the nuclear ribosomal genes of P. parumdentata were retrieved. Cells of the five morphotypes had identical 18S and 5.8S gene sequences. ITS1, ITS2 and 28S sequences produced two clusters: one grouping P. greenlandica and P. parumdentata and the other grouping P. jorgenseni, P. gigantea, and P. subrotundata. In contrast, CO1 nucleotide data yielded eight haplotypes clustered into five groups: one composed of P. greenlandica morphotypes, two distinct haplotypes of P. jorgenseni, and two distinct haplotypes of P. gigantea with one including P. subrotundata morphotypes. We investigated the co‐occurrence of different morphotypes in samples from sites across a large gradient of latitude and concentrations of Parafavella cells. Natural communities contained 2–6 different morphotypes. We conclude that both crypticity and polymorphism characterize Parafavella, as known for other tintinnid genera.