Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons
International audience In order to evaluate the efficiency of biostimulation of soil contaminated with oil hydrocarbons under sub-Antarctic conditions, a mesocosm study was initiated in May 2001 in the Kerguelen Archipelago (49°21¡¦S, 70°13¡¦E). The effects of temperature and fertilizer addition (In...
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Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2003
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Online Access: | https://hal.science/hal-02043879 https://hal.science/hal-02043879/document https://hal.science/hal-02043879/file/coulon_v58n4.pdf https://doi.org/10.2516/ogst:2003030 |
Summary: | International audience In order to evaluate the efficiency of biostimulation of soil contaminated with oil hydrocarbons under sub-Antarctic conditions, a mesocosm study was initiated in May 2001 in the Kerguelen Archipelago (49°21¡¦S, 70°13¡¦E). The effects of temperature and fertilizer addition (Inipol EAP-22, Elf Atochem) on soil bacterial assemblages contaminated with hydrocarbons were studied in 6-l batches of subantarctic soil incubated in the dark. Six different conditions were used at three temperatures (4, 10 and 20°C): control, fertilizer (50 ml), diesel oil (100 ml), diesel oil (100 ml) + fertilizer (50 ml), ¡§Arabian light¡¨ crude oil (100 ml) and crude oil (100 ml) + fertilizer (50 ml). Mesocosms were sampled on a regular basis over a seven-month period. All samples were analyzed for total bacteria, viable heterotrophic assemblages and hydrocarbon-utilising microflora. The results clearly showed a significant response of sub-Antarctic microbial soil communities to hydrocarbon contamination. Large increases in total, heterotrophic and hydrocarbon-utilising bacteria were observed (from less than 5 × 105 MPN g-1 to more than 108 MPN g-1 for hydrocarbon degrading bacteria). Temperature elevation had no significant impact on the total or heterotrophic assemblages but induced a one order of magnitude increase in hydrocarbon-utilising bacteria in contaminated mesocosms. In contrast, fertilizer addition had no clear effect on hydrocarbon-degrading specific bacteria but stimulated heterotrophic growth in diesel oil-contaminated soils. |
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