Tolerance of bivalve mollusc hemocytes to variable oxygen availability: a mitochondrial origin?

• Published in: Aquatic Living Resources
• Main Authors: Donaghy, Ludovic, Artigaud, Sébastien, Sussarellu, Rossana, Lambert, Christophe, Le Goïc, Nelly, Hegaret, Helene, Soudant, Philippe
• Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2013
• Subjects: Hemocyte; Hypoxia; Mitochondria; Reactive oxygen species; Flow cytometry; Oyster; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; [SDE.MCG]Environmental Sciences/Global Changes; [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology; [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health; [SDV.EE.ECO]Life Sciences [q-bio]/Ecology; environment/Ecosystems; [SDV.EE.IEO]Life Sciences [q-bio]/Ecology; environment/Symbiosis; [SDV.SA.STP]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://hal.science/hal-01154290; https://doi.org/10.1051/alr/2013054

International audience Bivalve molluscs survive lack of oxygen and may present cellular characteristics allowing tolerance tooxygen deprivation. The objective of the present work was to determine in vitro responses of circulating cells (i.e.,hemocytes) of Pacific oyster, Crassostrea gigas, to oxygen deprivation by analysis of mitochondrial membrane potential(ΔΨm) and reactive oxygen species (ROS) production. Hemocytes submitted to oxygen deprivation showed areduced ROS production and an increased ΔΨm. Confrontation of present results with recently reported findings allowsus to hypothesize the existence of an alternative mitochondrial reductase, and the involvement of mitochondrial potassiumchannel in maintenance of ΔΨm. Tolerance of bivalve hemocytes to variable oxygen availability appears, at leastpartially, related to mitochondrial specificities.