A cDNA microarray for Crassostrea virginica and C. gigas.

• Published in: Marine Biotechnology
• Main Authors: Jenny, Matthew J, Chapman, Robert W, Mancia, Annalaura, Chen, Yian A, Mckillen, David, J., Trent, Hal, Lang, Paul, Escoubas, Jean-Michel, Bachere, Evelyne, Boulo, Viviane, Liu, Z John, Gross, Paul S, Cunningham, Charles, Cupit, Pauline M, Tanguy, Arnaud, Guo, Ximing, Moraga, Dario, Boutet, Isabelle, Huvet, Arnaud, de Guise, Sylvain, Almeida, Jonas S, Warr, Gregory W
• Other Authors: Hollings Marine Laboratory, National Institute of Standards and Technology Gaithersburg (NIST), Biology Department (WHOI), Woods Hole Oceanographic Institution (WHOI), South Carolina Department of Natural Resources, Marine Resources Research Institute, Biochemistry and Molecular Biology, Medical University of South Carolina Charleston (MUSC), Department of Statistics, Texas AM University, Department of Fisheries and Wildlife, Oregon State University (OSU), Ecologie microbienne des insectes et interactions hôte-pathogène (EMIP), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Génome, populations, interactions, adaptation (GPIA), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Unité Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie (LEADNC), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Auburn University (AU), Department of Biology New Mexico, The University of New Mexico Albuquerque, Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff Roscoff (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Haskin Shellfish Research Laboratory (HSRL), Rutgers, The State University of New Jersey New Brunswick (RU), Rutgers University System (Rutgers)-Rutgers University System (Rutgers), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Physiologie et Ecophysiologie des Mollusques Marins (PE2M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Department of Pathobiology, University of Connecticut (UCONN), Program in Biomathematics and Biostatistics, The University of Texas Health Science Center at Houston (UTHealth), Department of Biochemistry and Molecular Biology
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2007
• Subjects: bivalve; Crassostrea gigas; Crassostrea virginica; heterologous hybridization; microarray; oyster; [SDE]Environmental Sciences; Pacific oyster
• Online Access: https://hal.univ-brest.fr/hal-00669982; https://doi.org/10.1007/s10126-007-9041-1

International audience The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496-feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis.