Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene
Juvenile turbot (Scophthalmus maximus) were injected intraperitonteally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled 1 and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metaboli...
Published in: | Marine Environmental Research |
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Main Authors: | , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2018
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Subjects: | |
Online Access: | https://repository.icr.ac.uk/handle/internal/2729 https://doi.org/10.1016/S0141-1136(02)00192-7 |
Summary: | Juvenile turbot (Scophthalmus maximus) were injected intraperitonteally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled 1 and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/108 nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P <0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 3OH BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/108 nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite–DNA interactions occurs indicative of other oxidative processes. |
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