Rodent hepatic helminths as components of hidden biodiversity

• Main Authors: Bjelić Čabrilo, Olivera, Čabrilo, Borislav, Miljević, Milan, Tošić, Božana, Bajić, Branka, Rajičić, Marija, Budinski, Ivana, Blagojević, Jelena
• Other Authors: Velevski, Metodija
• Format: Conference Object
• Language: English
• Published: Skopje: Macedonian Ecological Society 2022
• Subjects: Taenia crassiceps; T. martis; T. taeniaeformis; Calodium hepaticum; Microtus arvalis
• Online Access: http://radar.ibiss.bg.ac.rs/handle/123456789/5193; https://radar.ibiss.bg.ac.rs/bitstream/id/11387/6-congress-ecologists-Macedonia-50.pdf; https://hdl.handle.net/21.15107/rcub_ibiss_5193

Helminths represent hidden components of biodiversity in natural communities. Rather than elicit interest in their conservation, these endoparasitic worms tend to cause indifference or even disgust because of their appearance and way of life. However, they are present in every ecosystem and play a key role by integrating themselves into food webs and thereby securing their survival. Over the course of decades of research, 47 helminths were recorded in 10 rodent species in Serbia, with nematodes being the dominant group (29 species). Some helminths have high zoonotic potential due to their ability to infect humans, pets and domestic animals. This study focusses on hepatic helminths in a sample of six host species analysed over an eight-year period. Three tapeworm species and one nematode species were recorded, and their significance is broadly stated. A total of 770 wild rodents belonging to the species Apodemus flavicollis, A. agrarius, A. sylvaticus, Myodes glareolus, Microtus arvalis, and M. subterraneus were captured from 42 sites in Serbia from 2013-2021, dissected, and examined for cysts and lesions. For confirmation of parasite species, DNA was extracted and mitochondrial marker 12S rDNA was amplified and sequenced. Nematode eggs and larvae were detected based on histological slides made from the liver samples. The livers of 47 hosts contained cysts or visible lesions (6.1%). 12S rDNA fragment amplified successfully in 13 larval samples (1.68%), and three Taenia species were identified. Hydatigera (Taenia) taeniaeformis was dominant, followed by T. martis and T. crassiceps. No cysts were found in A. sylvaticus and M. subterraneus. Histological examination of the liver confirmed the presence of the nematode Calodium hepaticum in all three Apodemus host species, with a prevalence of 0.9%. Carnivores act as definitive hosts for all the registered tapeworm species: canids for T. crassiceps, felids for T. taeniaeformis (including domestic dogs and cats) and mustelids for T. martis. Rodents are intermediate ...