Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line
The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte tra...
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Online Access: | https://espace.inrs.ca/id/eprint/5384/ https://www.ncbi.nlm.nih.gov/pubmed/?term=28262249 https://doi.org/10.1016/j.marpolbul.2017.02.041 |
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ftinrsquebec:oai:espace.inrs.ca:5384 2023-05-15T16:33:03+02:00 Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line Kleinert, Christine Lacaze, Emilie Mounier, Meryl De Guise, Sylvain Fournier, Michel 2017-05-15 https://espace.inrs.ca/id/eprint/5384/ https://www.ncbi.nlm.nih.gov/pubmed/?term=28262249 https://doi.org/10.1016/j.marpolbul.2017.02.041 unknown Kleinert, Christine, Lacaze, Emilie, Mounier, Meryl, De Guise, Sylvain et Fournier, Michel (2017). Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line Marine Pollution Bulletin , vol. 118 , nº 1-2. p. 237-247. DOI:10.1016/j.marpolbul.2017.02.041 <https://doi.org/10.1016/j.marpolbul.2017.02.041>. doi:10.1016/j.marpolbul.2017.02.041 Cell line Harbor seal Immunotoxicity In vitro Mixture Pharmaceuticals Article Évalué par les pairs 2017 ftinrsquebec https://doi.org/10.1016/j.marpolbul.2017.02.041 2023-02-10T11:43:32Z The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte transformation was observed following an exposure to 12,500mug/L 17alpha-ethinyl estradiol and 25,000mug/L naproxen. Exposure to 12,500mug/L 17alpha-ethinyl estradiol decreased the percentage of cell in the G0/G1 phase of the cell cycle while increasing the percentage of cells in the S phase. Carbamazepine exposure increased the amount of cells in the G2/M phase. Binary mixtures showed synergistic effects in lymphocyte transformation, cell cycle and apoptosis assays. Concentrations inducing toxic effects in the cell line were similar to those affecting fish in previous studies. A reduction of functional activities of the immune system may lead to altered host resistance to pathogens in free-ranging pinnipeds. Article in Journal/Newspaper harbor seal Phoca vitulina Institut national de la recherche scientifique, Québec: Espace INRS Marine Pollution Bulletin 118 1-2 237 247 |
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Open Polar |
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Institut national de la recherche scientifique, Québec: Espace INRS |
op_collection_id |
ftinrsquebec |
language |
unknown |
topic |
Cell line Harbor seal Immunotoxicity In vitro Mixture Pharmaceuticals |
spellingShingle |
Cell line Harbor seal Immunotoxicity In vitro Mixture Pharmaceuticals Kleinert, Christine Lacaze, Emilie Mounier, Meryl De Guise, Sylvain Fournier, Michel Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line |
topic_facet |
Cell line Harbor seal Immunotoxicity In vitro Mixture Pharmaceuticals |
description |
The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte transformation was observed following an exposure to 12,500mug/L 17alpha-ethinyl estradiol and 25,000mug/L naproxen. Exposure to 12,500mug/L 17alpha-ethinyl estradiol decreased the percentage of cell in the G0/G1 phase of the cell cycle while increasing the percentage of cells in the S phase. Carbamazepine exposure increased the amount of cells in the G2/M phase. Binary mixtures showed synergistic effects in lymphocyte transformation, cell cycle and apoptosis assays. Concentrations inducing toxic effects in the cell line were similar to those affecting fish in previous studies. A reduction of functional activities of the immune system may lead to altered host resistance to pathogens in free-ranging pinnipeds. |
format |
Article in Journal/Newspaper |
author |
Kleinert, Christine Lacaze, Emilie Mounier, Meryl De Guise, Sylvain Fournier, Michel |
author_facet |
Kleinert, Christine Lacaze, Emilie Mounier, Meryl De Guise, Sylvain Fournier, Michel |
author_sort |
Kleinert, Christine |
title |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line |
title_short |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line |
title_full |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line |
title_fullStr |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line |
title_full_unstemmed |
Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line |
title_sort |
immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (phoca vitulina) b lymphoma cell line |
publishDate |
2017 |
url |
https://espace.inrs.ca/id/eprint/5384/ https://www.ncbi.nlm.nih.gov/pubmed/?term=28262249 https://doi.org/10.1016/j.marpolbul.2017.02.041 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_relation |
Kleinert, Christine, Lacaze, Emilie, Mounier, Meryl, De Guise, Sylvain et Fournier, Michel (2017). Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line Marine Pollution Bulletin , vol. 118 , nº 1-2. p. 237-247. DOI:10.1016/j.marpolbul.2017.02.041 <https://doi.org/10.1016/j.marpolbul.2017.02.041>. doi:10.1016/j.marpolbul.2017.02.041 |
op_doi |
https://doi.org/10.1016/j.marpolbul.2017.02.041 |
container_title |
Marine Pollution Bulletin |
container_volume |
118 |
container_issue |
1-2 |
container_start_page |
237 |
op_container_end_page |
247 |
_version_ |
1766022768568041472 |