Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line
The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte tra...
| Published in: | Marine Pollution Bulletin |
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| Main Authors: | , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://espace.inrs.ca/id/eprint/5384/ https://www.ncbi.nlm.nih.gov/pubmed/?term=28262249 https://doi.org/10.1016/j.marpolbul.2017.02.041 |
| Summary: | The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte transformation was observed following an exposure to 12,500mug/L 17alpha-ethinyl estradiol and 25,000mug/L naproxen. Exposure to 12,500mug/L 17alpha-ethinyl estradiol decreased the percentage of cell in the G0/G1 phase of the cell cycle while increasing the percentage of cells in the S phase. Carbamazepine exposure increased the amount of cells in the G2/M phase. Binary mixtures showed synergistic effects in lymphocyte transformation, cell cycle and apoptosis assays. Concentrations inducing toxic effects in the cell line were similar to those affecting fish in previous studies. A reduction of functional activities of the immune system may lead to altered host resistance to pathogens in free-ranging pinnipeds. |
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