Myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli

Myostatin and hypoxia signalling pathways are able to induce skeletal muscle atrophy, but whether a relationship between these two pathways exists is currently unknown. Here, we tested the hypothesis that a potential mechanism for hypoxia effect on skeletal muscle may be through regulation of myosta...

Full description

Bibliographic Details
Published in:Molecular and Cellular Endocrinology
Main Authors: Hayot, Maurice, Rodriguez, Julie, Vernus, Barbara, Carnac, Gilles, Jean, Elise, Allen, David, Goret, Lucie, Obert, Philippe, Candau, Robin, Bonnieu, Anne
Format: Article in Journal/Newspaper
Language:English
Published: 2011
Subjects:
HYPOXIC STIMULUS;MYOSTATIN;SKELETAL MUSCLE ATROPHY;COPD;MYOTUBES ;RAT;MALADIE PULMONAIRE OBSTRUCTIVE CHRONIQUE
rattus rattus
anticorps
maladie respiratoire
maladie génétique
atrophie musculaire
hypoxie
myostatine
Rattus rattus
Online Access:http://prodinra.inra.fr/ft/131994A2-EDF6-4E94-B41C-DB874D2D7DC8
http://prodinra.inra.fr/record/45915
https://doi.org/10.1016/j.mce.2010.09.008
Description
Summary:Myostatin and hypoxia signalling pathways are able to induce skeletal muscle atrophy, but whether a relationship between these two pathways exists is currently unknown. Here, we tested the hypothesis that a potential mechanism for hypoxia effect on skeletal muscle may be through regulation of myostatin. We reported an induction of myostatin expression in muscles of rats exposed to chronic hypoxia. Interestingly, we also demonstrated increased skeletal muscle myostatin protein expression in skeletal muscle of hypoxemic patients with severe chronic obstructive pulmonary disease (COPD). Parallel studies in human skeletal muscle cell cultures showed that induction of myostatin expression in myotubes treated with hypoxia-mimicking agent such as cobalt chloride (CoCl2) is associated with myotube atrophy. Furthermore, we demonstrated that inhibition of myostatin by means of genetic deletion of myostatin or treatment with blocking antimyostatin antibodies inhibits the CoCl2-induced atrophy in muscle cells. Finally, addition of recombinant myostatin restored the CoCl2-induced atrophy in myostatin deficient myotubes. These results strongly suggest that myostatin can play an essential role in the adaptation of skeletal muscle to hypoxic environment

Similar Items