Remodeling of the cycling transcriptome of the oyster Crassostrea gigas by the harmful algae Alexandrium minutum

As a marine organism, the oyster Crassostrea gigas inhabits a complex biotope governed by interactions between the moon and the sun cycles. We used next-generation sequencing to investigate temporal regulation of oysters under light/dark entrainment and the impact of harmful algal exposure. We found...

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Bibliographic Details
Published in:Scientific Reports
Main Authors: Payton, Laura, Perrigault, Mickael, Hoede, Claire, Massabuau, Jean-Charles, Sow, Mohamedou, Huvet, Arnaud, Boullot, Floriane, Fabioux, Caroline, Hegaret, Helene, Tran, Damien
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
MAMMALIAN CIRCADIAN CLOCK;DIFFERENTIAL EXPRESSION ANALYSIS;PARALYTIC SHELLFISH TOXINS;GENE-EXPRESSION;SUPRACHIASMATIC NUCLEUS;FEEDING RESPONSES;MOUSE-LIVER;SHIFT WORK;DINOFLAGELLATE;RHYTHMS
Crassostrea gigas
Online Access:http://prodinra.inra.fr/ft/E61F1065-F192-4870-8F95-65EC3FEEF8E7
http://prodinra.inra.fr/record/397675
https://doi.org/10.1038/s41598-017-03797-4
Description
Summary:As a marine organism, the oyster Crassostrea gigas inhabits a complex biotope governed by interactions between the moon and the sun cycles. We used next-generation sequencing to investigate temporal regulation of oysters under light/dark entrainment and the impact of harmful algal exposure. We found that approximate to 6% of the gills' transcriptome exhibits circadian expression, characterized by a nocturnal and bimodal pattern. Surprisingly, a higher number of ultradian transcripts were also detected under solely circadian entrainment. The results showed that a bloom of Alexandrium minutum generated a remodeling of the bivalve's temporal structure, characterized by a loss of oscillations, a genesis of de novo oscillating transcripts, and a switch in the period of oscillations. These findings provide unprecedented insights into the diurnal landscape of the oyster's transcriptome and pleiotropic remodeling due to toxic algae exposure, revealing the intrinsic plasticity of the cycling transcriptome in oysters.

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