The effect of the concentration of calcium ions and pH on the milkclotting activity of an engineered variant of recombinant reindeer chymosin (Rangifer tarandus)

The effect of active acidity and calcium chloride concentration on the milk coagulation rate under the action of an engineered variant of recombinant reindeer chymosin (Rangifer tarandus) with a point amino acid replacement Lys53→Glu, was studied. It was found that the dependence of milk-clotting ac...

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Bibliographic Details
Published in:Cheesemaking and buttermaking
Main Authors: Pushkarev Vladimir, Musina Olga, Belenkaya Svetlana, Shcherbakov Dmitry, Koval Anatoliy, Belov Aleksandr, Elchaninov Vadim
Format: Text
Language:Russian
Published: Kemerovo State University 2024
Subjects:
Rangifer tarandus
Online Access:https://doi.org/10.31515/2073-4018-2023-2-31-33
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Summary:The effect of active acidity and calcium chloride concentration on the milk coagulation rate under the action of an engineered variant of recombinant reindeer chymosin (Rangifer tarandus) with a point amino acid replacement Lys53→Glu, was studied. It was found that the dependence of milk-clotting activity on pH and calcium chloride concentration of engineered recombinant reindeer chymosin is comparable to high quality commercial genetically engineered milk coagulants.

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