Milk-clotting activity and general proteolytic activity of an engineered variant of recombinant reindeer chymosin (Rangifer tarandus)
The milk-clotting and general proteolytic activity of an engineered variant of recombinant reindeer chymosin (Rangifer tarandus) with a point amino acid replacement Lys53→Glu was studied. Commercial recombinant cow and single-humped camel chymosins were used as comparison enzymes. It is shown that t...
| Published in: | Cheesemaking and buttermaking |
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| Main Authors: | , , , , , , |
| Format: | Text |
| Language: | Russian |
| Published: |
Kemerovo State University
2024
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| Subjects: | |
| Online Access: | https://doi.org/10.31515/2073-4018-2023-1-22-25 |
| Summary: | The milk-clotting and general proteolytic activity of an engineered variant of recombinant reindeer chymosin (Rangifer tarandus) with a point amino acid replacement Lys53→Glu was studied. Commercial recombinant cow and single-humped camel chymosins were used as comparison enzymes. It is shown that the total proteolytic activity of the engineered variant of reindeer chymosin is 36 % lower than that of recombinant cow chymosin. The engineered version of recombinant reindeer chymosin was characterized by extremely low relative milk-clotting activity, which led to a decrease in its specificity, compared with recombinant cow and camel chymosins by 2,4 and 9,8 times respectively. |
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