Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments
Global warming induced sea ice loss increases Arctic maritime traffic, enhancing the risk of ecosystem contamination from fuel spills and nutrient loading. The impact of marine diesel on bacterial metabolic activity and diversity, assessed by colorimetric assay, 16S rRNA and metagenomic sequencing,...
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Oxford, PERGAMON-ELSEVIER SCIENCE LTD
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Online Access: | http://infoscience.epfl.ch/record/304657 https://doi.org/10.1016/j.marpolbul.2023.115226 |
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ftinfoscience:oai:infoscience.epfl.ch:304657 2023-10-09T21:48:23+02:00 Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments Durand, Margaux Touchette, David Chen, Ya-Jou Magnuson, Elisse Wasserscheid, Jessica Greer, Charles W. Whyte, Lyle G. Altshuler, Ianina 2023-08-28T00:46:29Z http://infoscience.epfl.ch/record/304657 https://doi.org/10.1016/j.marpolbul.2023.115226 unknown Oxford, PERGAMON-ELSEVIER SCIENCE LTD http://infoscience.epfl.ch/record/304657 isi:001046492500001 doi:10.1016/j.marpolbul.2023.115226 http://infoscience.epfl.ch/record/304657 Text 2023 ftinfoscience https://doi.org/10.1016/j.marpolbul.2023.115226 2023-09-10T23:52:46Z Global warming induced sea ice loss increases Arctic maritime traffic, enhancing the risk of ecosystem contamination from fuel spills and nutrient loading. The impact of marine diesel on bacterial metabolic activity and diversity, assessed by colorimetric assay, 16S rRNA and metagenomic sequencing, of Northwest Passage (Arctic Ocean) beach sediments was assessed with nutrient amendment at environmentally relevant temperatures (5 and 15 degrees C). Higher temperature and nutrients stimulated microbial activity, while diesel reduced it, with metabolism inhibited at and above 0.01 % (without nutrients) and at 1 % (with nutrients) diesel inclusions. Diesel exposure significantly decreased microbial diversity and selected for Psychrobacter genus. Microbial hydrocarbon degradation, organic compound metabolism, and exopolysaccharide production gene abundances increased under higher diesel concentrations. Metagenomic binning recovered nine MAGs/bins with hydrocarbon degradation genes. We demonstrate a nutrients' rescue-type effect in diesel contaminated microbial communities via enrichment of microorganisms with stress response, aromatic compound, and ammonia assimilation metabolisms. Text Arctic Arctic Ocean Global warming Northwest passage Sea ice EPFL Infoscience (Ecole Polytechnique Fédérale Lausanne) Arctic Arctic Ocean Northwest Passage Marine Pollution Bulletin 194 115226 |
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Open Polar |
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EPFL Infoscience (Ecole Polytechnique Fédérale Lausanne) |
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ftinfoscience |
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unknown |
description |
Global warming induced sea ice loss increases Arctic maritime traffic, enhancing the risk of ecosystem contamination from fuel spills and nutrient loading. The impact of marine diesel on bacterial metabolic activity and diversity, assessed by colorimetric assay, 16S rRNA and metagenomic sequencing, of Northwest Passage (Arctic Ocean) beach sediments was assessed with nutrient amendment at environmentally relevant temperatures (5 and 15 degrees C). Higher temperature and nutrients stimulated microbial activity, while diesel reduced it, with metabolism inhibited at and above 0.01 % (without nutrients) and at 1 % (with nutrients) diesel inclusions. Diesel exposure significantly decreased microbial diversity and selected for Psychrobacter genus. Microbial hydrocarbon degradation, organic compound metabolism, and exopolysaccharide production gene abundances increased under higher diesel concentrations. Metagenomic binning recovered nine MAGs/bins with hydrocarbon degradation genes. We demonstrate a nutrients' rescue-type effect in diesel contaminated microbial communities via enrichment of microorganisms with stress response, aromatic compound, and ammonia assimilation metabolisms. |
format |
Text |
author |
Durand, Margaux Touchette, David Chen, Ya-Jou Magnuson, Elisse Wasserscheid, Jessica Greer, Charles W. Whyte, Lyle G. Altshuler, Ianina |
spellingShingle |
Durand, Margaux Touchette, David Chen, Ya-Jou Magnuson, Elisse Wasserscheid, Jessica Greer, Charles W. Whyte, Lyle G. Altshuler, Ianina Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments |
author_facet |
Durand, Margaux Touchette, David Chen, Ya-Jou Magnuson, Elisse Wasserscheid, Jessica Greer, Charles W. Whyte, Lyle G. Altshuler, Ianina |
author_sort |
Durand, Margaux |
title |
Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments |
title_short |
Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments |
title_full |
Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments |
title_fullStr |
Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments |
title_full_unstemmed |
Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments |
title_sort |
effects of marine diesel on microbial diversity and activity in high arctic beach sediments |
publisher |
Oxford, PERGAMON-ELSEVIER SCIENCE LTD |
publishDate |
2023 |
url |
http://infoscience.epfl.ch/record/304657 https://doi.org/10.1016/j.marpolbul.2023.115226 |
geographic |
Arctic Arctic Ocean Northwest Passage |
geographic_facet |
Arctic Arctic Ocean Northwest Passage |
genre |
Arctic Arctic Ocean Global warming Northwest passage Sea ice |
genre_facet |
Arctic Arctic Ocean Global warming Northwest passage Sea ice |
op_source |
http://infoscience.epfl.ch/record/304657 |
op_relation |
http://infoscience.epfl.ch/record/304657 isi:001046492500001 doi:10.1016/j.marpolbul.2023.115226 |
op_doi |
https://doi.org/10.1016/j.marpolbul.2023.115226 |
container_title |
Marine Pollution Bulletin |
container_volume |
194 |
container_start_page |
115226 |
_version_ |
1779311475464077312 |