Effects of marine diesel on microbial diversity and activity in high Arctic beach sediments

Global warming induced sea ice loss increases Arctic maritime traffic, enhancing the risk of ecosystem contamination from fuel spills and nutrient loading. The impact of marine diesel on bacterial metabolic activity and diversity, assessed by colorimetric assay, 16S rRNA and metagenomic sequencing,...

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Bibliographic Details
Published in:Marine Pollution Bulletin
Main Authors: Durand, Margaux, Touchette, David, Chen, Ya-Jou, Magnuson, Elisse, Wasserscheid, Jessica, Greer, Charles W., Whyte, Lyle G., Altshuler, Ianina
Format: Text
Language:unknown
Published: Oxford, PERGAMON-ELSEVIER SCIENCE LTD 2023
Subjects:
Arctic
Arctic Ocean
Northwest Passage
Global warming
Northwest passage
Sea ice
Online Access:http://infoscience.epfl.ch/record/304657
https://doi.org/10.1016/j.marpolbul.2023.115226
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Summary:Global warming induced sea ice loss increases Arctic maritime traffic, enhancing the risk of ecosystem contamination from fuel spills and nutrient loading. The impact of marine diesel on bacterial metabolic activity and diversity, assessed by colorimetric assay, 16S rRNA and metagenomic sequencing, of Northwest Passage (Arctic Ocean) beach sediments was assessed with nutrient amendment at environmentally relevant temperatures (5 and 15 degrees C). Higher temperature and nutrients stimulated microbial activity, while diesel reduced it, with metabolism inhibited at and above 0.01 % (without nutrients) and at 1 % (with nutrients) diesel inclusions. Diesel exposure significantly decreased microbial diversity and selected for Psychrobacter genus. Microbial hydrocarbon degradation, organic compound metabolism, and exopolysaccharide production gene abundances increased under higher diesel concentrations. Metagenomic binning recovered nine MAGs/bins with hydrocarbon degradation genes. We demonstrate a nutrients' rescue-type effect in diesel contaminated microbial communities via enrichment of microorganisms with stress response, aromatic compound, and ammonia assimilation metabolisms.

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