Occurrence and expression of cspA, a cold shock gene, in Antarctic psychrotropic bacteria
The homologue of cold shock gene cspA of Escherichia coli was detected in various isolates of Antarctic psychrotrophs representing both Gram-positive and Gram-negative bacteria. The Northern hybridization study indicated that the transcript size of cspA in the psychrotrophic Gram-positive bacterium...
| Main Authors: | , , , |
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| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
John Wiley and Sons
1994
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| Subjects: | |
| Online Access: | http://repository.ias.ac.in/64321/ http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1994.tb06675.x/abstract |
| Summary: | The homologue of cold shock gene cspA of Escherichia coli was detected in various isolates of Antarctic psychrotrophs representing both Gram-positive and Gram-negative bacteria. The Northern hybridization study indicated that the transcript size of cspA in the psychrotrophic Gram-positive bacterium Arthrobacter protophormiae and Gram-negative Pseudomonas fluorescens was similar to that of E. coli and that the cspA homologues in these two psychrotrophs were expressed constitutively at a low level both at 4°C and 22°C. In P. fluorescens, the expression of cspA mRNA was inducible after shift of temperature from 22 to 4°C and the maximum level of induction occurred after 1 h which correlated with the time-lag required for growth of the culture after temperature shift. |
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