Preliminary results of different methods of artificial fertilization with fresh sperm of turbot (Scophthalmus maximus L.)

• Published in: Aquaculture Research
• Main Authors: Chereguini-Fernández-Maquieira, M.O. (María del Olvido), Peleteiro-Alonso, J.B. (José Benito), Cal, R. (Rosa), García, A.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Centro Oceanográfico de Santander 1995
• Subjects: Scophthalmus maximus; Turbot
• Online Access: http://hdl.handle.net/10508/6806; https://doi.org/10.1046/j.1365-2109.1999.00326.x

With the aim of improving artificial fertilization (AF) in turbot, Scophthalmus maximus (L.), a series of fertilization experiments was carried out under dry conditions and different wet conditions (eggs/sea water: 2V/V and V/V). Another series of fertilization experiments was carried out with different quantities of sperm pool to determine the optimal ratio of spermatozoa to eggs for each AF method. Sperm pool from two males and eggs from spawns with a viability rate of > 70% were used. The sperm pool’s density (0.4–5.18 × 109 sperm mL–1) and motility (1–5) had been assessed previously. Significantly different fertilization rates were found when comparing 2V/V and V/V wet conditions. Significantly higher fertilization rates were found in dry fertilization when the sperm–egg ratio was > 9000 spermatozoa per egg and, under wet condition V/V, at 3000–4000 spermatozoa per egg. 0