Rapid flow cytometry method for triploidy determination in turbot (Scopthalmus maximus L.)
Accurate methods for assessing triploidy are important because treatments designed to induce poliploidy are not 100% and offspring from treated eggs are usually a mixture of triploids and diploids. Flow cytometry was more recently applied to triploidy determination and usually carried out using eryt...
| Main Authors: | , , , , |
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| Format: | Other/Unknown Material |
| Language: | English |
| Published: |
Centro Oceanográfico de Santander
2012
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10508/529 |
| Summary: | Accurate methods for assessing triploidy are important because treatments designed to induce poliploidy are not 100% and offspring from treated eggs are usually a mixture of triploids and diploids. Flow cytometry was more recently applied to triploidy determination and usually carried out using erythrocytes. This paper describes a rapid and simple method for triploidy determination from the larval stage of turbot (Scopthalmus maximus) by flow cytometry. High-quality histograms were obtained from turbot larvae and the very good reproductibility provided unambigous discrimination of diploids and triploids even in early stages development. |
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