Sexing European rabbits (Oryctolagus cuniculus), European brown hares (Lepus europaeus) and mountain hares (Lepus timidus) with ZFX and ZFY loci

We describe a new method for the sex determination of tissue originating from Oryctolagus cuniculus (European rabbit), Lepus europaeus (European brown hare) and Lepus timidus (mountain hare) based on PCR-RFLP analysis of point mutations that differentiate the ZFX and ZFY gene sequences. Among severa...

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Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: Fontanesi, L., Tazzoli, M., Robinson, T. J., Russo, V., Pecchioli, Elena, Hauffe, Heidi Christine
Other Authors: Pecchioli, E., Hauffe, H.C., Robinson, T.J.
Format: Article in Journal/Newspaper
Language:English
Published: 2008
Subjects:
Hare
Leporidae
Molecular sexing
Rabbit
ZFX
ZFY
Lepus timidus
mountain hare
Online Access:http://hdl.handle.net/10449/20475
https://doi.org/10.1111/j.1755-0998.2008.02167.x
Description
Summary:We describe a new method for the sex determination of tissue originating from Oryctolagus cuniculus (European rabbit), Lepus europaeus (European brown hare) and Lepus timidus (mountain hare) based on PCR-RFLP analysis of point mutations that differentiate the ZFX and ZFY gene sequences. Among several applications, this PCR-RFLP method could be used to investigate gender ratio and evaluate the population dynamics of these species using samples collected when sex cannot be identified.

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