Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

• Published in: Macromolecular Rapid Communications
• Main Authors: Miletic, N., Abetz, V., Ebert, K., Loos, K.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2010
• Subjects: info:eu-repo/classification/ddc/620.11; Antarc*; Antarctica
• Online Access: https://publications.hereon.de/id/27602; https://publications.hzg.de/id/27602; http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2009/Miletic-macrapcom.pdf

Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435.