Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles
Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobi...
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fthzgzmk:oai:publications.hereon.de:27602 2023-06-11T04:05:49+02:00 Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles Miletic, N. Abetz, V. Ebert, K. Loos, K. 2010 https://publications.hereon.de/id/27602 https://publications.hzg.de/id/27602 http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2009/Miletic-macrapcom.pdf en eng Wiley http://dx.doi.org/10.1002/marc.200900497 urn:issn:1022-1336 https://publications.hereon.de/id/27602 https://publications.hzg.de/id/27602 http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2009/Miletic-macrapcom.pdf info:eu-repo/semantics/openAccess open_access oa_gruen issn:1022-1336 Miletic, N.; Abetz, V.; Ebert, K.; Loos, K.: Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles. In: Macromolecular Rapid Communications. Vol. 31 (2010) 1, 71 - 74. (DOI:10.1002/marc.200900497) info:eu-repo/classification/ddc/620.11 info:eu-repo/semantics/article Zeitschrift Artikel 2010 fthzgzmk https://doi.org/10.1002/marc.200900497 2023-05-28T23:23:02Z Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435. Article in Journal/Newspaper Antarc* Antarctica Hereon Publications (Helmholtz-Zentrum) Macromolecular Rapid Communications 31 1 71 74 |
institution |
Open Polar |
collection |
Hereon Publications (Helmholtz-Zentrum) |
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fthzgzmk |
language |
English |
topic |
info:eu-repo/classification/ddc/620.11 |
spellingShingle |
info:eu-repo/classification/ddc/620.11 Miletic, N. Abetz, V. Ebert, K. Loos, K. Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
topic_facet |
info:eu-repo/classification/ddc/620.11 |
description |
Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435. |
format |
Article in Journal/Newspaper |
author |
Miletic, N. Abetz, V. Ebert, K. Loos, K. |
author_facet |
Miletic, N. Abetz, V. Ebert, K. Loos, K. |
author_sort |
Miletic, N. |
title |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_short |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_full |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_fullStr |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_full_unstemmed |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_sort |
immobilization of candida antarctica lipase b on polystyrene nanoparticles |
publisher |
Wiley |
publishDate |
2010 |
url |
https://publications.hereon.de/id/27602 https://publications.hzg.de/id/27602 http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2009/Miletic-macrapcom.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
issn:1022-1336 Miletic, N.; Abetz, V.; Ebert, K.; Loos, K.: Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles. In: Macromolecular Rapid Communications. Vol. 31 (2010) 1, 71 - 74. (DOI:10.1002/marc.200900497) |
op_relation |
http://dx.doi.org/10.1002/marc.200900497 urn:issn:1022-1336 https://publications.hereon.de/id/27602 https://publications.hzg.de/id/27602 http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2009/Miletic-macrapcom.pdf |
op_rights |
info:eu-repo/semantics/openAccess open_access oa_gruen |
op_doi |
https://doi.org/10.1002/marc.200900497 |
container_title |
Macromolecular Rapid Communications |
container_volume |
31 |
container_issue |
1 |
container_start_page |
71 |
op_container_end_page |
74 |
_version_ |
1768377475862102016 |