Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles
Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobi...
| Published in: | Macromolecular Rapid Communications |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2010
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| Subjects: | |
| Online Access: | https://publications.hereon.de/id/27602 https://publications.hzg.de/id/27602 http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2009/Miletic-macrapcom.pdf |
| Summary: | Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435. |
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