Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis
Total selenium content and the selenium distribution in the soluble and insoluble protein-bound fractions obtained after aqueous extraction and centrifugation of Antarctic krill samples were determined. About 26% of the total selenium ( Se) (2.4 μg/g dry weight) was found in the supernatant and the...
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fthzgzmk:oai:publications.hereon.de:23203 2023-06-11T04:06:49+02:00 Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis Siwek, M. Galunsky, B. Niemeyer, B. 2005 https://publications.hereon.de/id/23203 https://publications.hzg.de/id/23203 en eng http://dx.doi.org/10.1007/s00216-004-2936-z urn:issn:1618-2642 https://publications.hereon.de/id/23203 https://publications.hzg.de/id/23203 info:eu-repo/semantics/closedAccess issn:1618-2642 Siwek, M.; Galunsky, B.; Niemeyer, B.: Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis. In: Analytical and Bioanalytical Chemistry. Vol. 381 (2005) 3, 737 - 741. (DOI:10.1007/s00216-004-2936-z ) info:eu-repo/classification/ddc/551 info:eu-repo/semantics/article Zeitschrift Artikel 2005 fthzgzmk https://doi.org/10.1007/s00216-004-2936-z 2023-05-28T23:22:13Z Total selenium content and the selenium distribution in the soluble and insoluble protein-bound fractions obtained after aqueous extraction and centrifugation of Antarctic krill samples were determined. About 26% of the total selenium ( Se) (2.4 μg/g dry weight) was found in the supernatant and the rest was in the pellet. The digestion of the protein and the isolation of low molecular selenium-containing fractions was achieved by enzymatic hydrolysis followed by size-exclusion chromatography in conjunction with graphite furnace atomic absorption spectrometry. From the applied various proteinases (trypsin, -chymotrypsin, subtilisin, pronase E), the treatment with pronase E led to best recovery of the total Se. About 96% of the total selenium was found in the hydrolysate, mainly in low molecular weight fractions. 15% of the low molecular weight Se-species were in fractions with molecular weights in the range of amino acids. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Hereon Publications (Helmholtz-Zentrum) Antarctic Analytical and Bioanalytical Chemistry 381 3 737 741 |
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Hereon Publications (Helmholtz-Zentrum) |
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English |
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info:eu-repo/classification/ddc/551 |
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info:eu-repo/classification/ddc/551 Siwek, M. Galunsky, B. Niemeyer, B. Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis |
topic_facet |
info:eu-repo/classification/ddc/551 |
description |
Total selenium content and the selenium distribution in the soluble and insoluble protein-bound fractions obtained after aqueous extraction and centrifugation of Antarctic krill samples were determined. About 26% of the total selenium ( Se) (2.4 μg/g dry weight) was found in the supernatant and the rest was in the pellet. The digestion of the protein and the isolation of low molecular selenium-containing fractions was achieved by enzymatic hydrolysis followed by size-exclusion chromatography in conjunction with graphite furnace atomic absorption spectrometry. From the applied various proteinases (trypsin, -chymotrypsin, subtilisin, pronase E), the treatment with pronase E led to best recovery of the total Se. About 96% of the total selenium was found in the hydrolysate, mainly in low molecular weight fractions. 15% of the low molecular weight Se-species were in fractions with molecular weights in the range of amino acids. |
format |
Article in Journal/Newspaper |
author |
Siwek, M. Galunsky, B. Niemeyer, B. |
author_facet |
Siwek, M. Galunsky, B. Niemeyer, B. |
author_sort |
Siwek, M. |
title |
Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis |
title_short |
Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis |
title_full |
Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis |
title_fullStr |
Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis |
title_full_unstemmed |
Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis |
title_sort |
isolation of selenium organic species from antarctic krill after enzymatic hydrolysis |
publishDate |
2005 |
url |
https://publications.hereon.de/id/23203 https://publications.hzg.de/id/23203 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill |
genre_facet |
Antarc* Antarctic Antarctic Krill |
op_source |
issn:1618-2642 Siwek, M.; Galunsky, B.; Niemeyer, B.: Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis. In: Analytical and Bioanalytical Chemistry. Vol. 381 (2005) 3, 737 - 741. (DOI:10.1007/s00216-004-2936-z ) |
op_relation |
http://dx.doi.org/10.1007/s00216-004-2936-z urn:issn:1618-2642 https://publications.hereon.de/id/23203 https://publications.hzg.de/id/23203 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s00216-004-2936-z |
container_title |
Analytical and Bioanalytical Chemistry |
container_volume |
381 |
container_issue |
3 |
container_start_page |
737 |
op_container_end_page |
741 |
_version_ |
1768378995899891712 |