Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis
Total selenium content and the selenium distribution in the soluble and insoluble protein-bound fractions obtained after aqueous extraction and centrifugation of Antarctic krill samples were determined. About 26% of the total selenium ( Se) (2.4 μg/g dry weight) was found in the supernatant and the...
| Published in: | Analytical and Bioanalytical Chemistry |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
2005
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| Subjects: | |
| Online Access: | https://publications.hereon.de/id/23203 https://publications.hzg.de/id/23203 |
| Summary: | Total selenium content and the selenium distribution in the soluble and insoluble protein-bound fractions obtained after aqueous extraction and centrifugation of Antarctic krill samples were determined. About 26% of the total selenium ( Se) (2.4 μg/g dry weight) was found in the supernatant and the rest was in the pellet. The digestion of the protein and the isolation of low molecular selenium-containing fractions was achieved by enzymatic hydrolysis followed by size-exclusion chromatography in conjunction with graphite furnace atomic absorption spectrometry. From the applied various proteinases (trypsin, -chymotrypsin, subtilisin, pronase E), the treatment with pronase E led to best recovery of the total Se. About 96% of the total selenium was found in the hydrolysate, mainly in low molecular weight fractions. 15% of the low molecular weight Se-species were in fractions with molecular weights in the range of amino acids. |
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