Upotreba patočnog ulja, nusproizvoda prehrambene industrije u biotehnologiji - Kinetički model enzimske esterifikacije izoamilnog alkohola s oleinskom kiselinom pomoću lipaze B iz plijesni Candida antarctica

• Main Authors: Nemestóthy, Nándor; Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary; [e-mail], Gubicza, László; Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary, Fehér, Erika; Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary, Bélafi-Bakó, Katalin; Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary
• Format: Text
• Language: English
• Published: Faculty of Food Technology and Biotechnology, University of Zagreb; [e-mail] 2008
• Subjects: patočno ulje; esterifikacija; lipaza; ping-pong bi-bi mehanizam; fusel oil; esterification; lipase; ping-pong bi-bi mechanism; Antarc*; Antarctica
• Online Access: http://hrcak.srce.hr/22214; http://hrcak.srce.hr/file/34893

Patočno je ulje („fusel oil“) nusproizvod destilacije, a njegov je glavni sastojak izoamilni alkohol čijom esterifikacijom nastaju esteri. U ovom je radu ispitana esterifikacija oleinske kiseline s izoamilnim alkoholom u n-heptanu pomoću lipaze B izolirane iz kvasca Candida antarctica (preparat Novozym 435). Primijenjen je složeni kinetički model ping-pong bi-bi mehanizma, pri čemu je uzeta u obzir pojava inhibicije. Parametri modela određeni su numeričkim metodama. Utvrđeno je da je za rezultate pokusa i opis enzimske reakcije najprikladniji model sa 4 parametra. Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and described properly the enzymatic reaction.