北海道のヒグマ (Ursus arctos yesoensis) の遺伝的変異に関する研究
there was no difference in the condition of immobilized bears between the two combinations. ln use of tiletamine-zolazepam, there was no difference in the volume and induction time between sexes. The tiletamine-zolazepam was found to be more effective for the immobilization of Hokkaido brown bears....
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| Format: | Other/Unknown Material |
| Language: | Japanese |
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Hokkaido University
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| Online Access: | http://hdl.handle.net/2115/51271 https://doi.org/10.11501/3082681 |
| Summary: | there was no difference in the condition of immobilized bears between the two combinations. ln use of tiletamine-zolazepam, there was no difference in the volume and induction time between sexes. The tiletamine-zolazepam was found to be more effective for the immobilization of Hokkaido brown bears. 2. Allozyme analysis of genetic differentiation A total of 21 biochemical loci were analyzed electrophoretically in 10 bears from 3 subpopulations in Hokkaido in order to estimate genetic variability of the brown bear population. No polymorp hic locus was observed and the genetic variability of Hokkaido brown bears are thought to be as low as other land mammals inhabiting in Japan. 3. Application of DNA fingerprinting in bears. DNA fingerprinting employing a minisatellite Myo probe, which is known to be valuable in forensic study, was applied for individual identification and paternity determination of 47 captive bears. Moreover, it was applied for the estimation of genetic variability within and between subpopulations using 6 captive bears with known origins and 26 free-ranging bears captured in the southwestern Oshima peninsula (n=13) and the Shiretoko peninsula (n=13). Two restriction enzymes, HinfI and HaeIII were used to make DNA fingerprints and the band patterns obtained from 45 (HinfI) and 33 (HaeIII) pairs of randomly selected bears were compared with each other. The obtained values of the probability x that a fragment in an individual is also present in the other were 0.69 for HinfI and 0.83 for HaeIII and the mean probability of all fragments was calculated to be 2.5×10-2 for HinfI and 1.3×10-1 for HaeIII. The value of x for HinfI (0.69) was similar to that obtained from other species, such as domestic animals. Thus, the results suggest that DNA fingerprinting can be used for individual identification. On the other hand, DNA fingerprinting is effective for paternity determination only in the case suspected males are limited, owing to the insufficient number of paternal fragments. The estimates of the ... |
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