Artificial hybridization of Japanese and European eel (Anguilla japonica x A. anguilla) by using cryopreserved sperm from freshwater reared males

Eggs of Japanese eel (Anguilla anguilla) were fertilized with cryopreserved sperm of European eel (A. anguilla) obtained from fish reared and matured in freshwater conditions. A new genomic PCR-RFLP marker was developed to distinguish the two species and detect maternally and paternally derived DNA...

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Bibliographic Details
Published in:Aquaculture
Main Authors: Müller, T., Horváth, Á., Takahashi, E., Kolics, B., Bakos, K., Decsi, K., Kovács, B., Taller, J., Urbányi, B., Bercsényi, M., Horváth, L., Adachi, S., Arai, K., Yamaha, E.
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier B.V.
Subjects:
Propagation
Fertilization
Eel larvae
Hybrid
PCR-RFLP
FSH
666
Anguilla anguilla
European eel
Online Access:http://hdl.handle.net/2115/49518
https://doi.org/10.1016/j.aquaculture.2012.04.007
Description
Summary:Eggs of Japanese eel (Anguilla anguilla) were fertilized with cryopreserved sperm of European eel (A. anguilla) obtained from fish reared and matured in freshwater conditions. A new genomic PCR-RFLP marker was developed to distinguish the two species and detect maternally and paternally derived DNA fragments to genetically verify the hybrid nature of the offspring.

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