Anticoagulation, Centrifligation Time, and Sample Replicate Number in the Microhematocrit Method for Avian Blood
THE determination of hematocrit (Hct) is commonly employed as a measure of the erythrocyte concentration in the blood. Most studies in avian hematology have employed macro Hct methods, requiring at least 1 ml. of blood and centrifugation for 30 min. or more. There has, however, been increasing adopt...
| Published in: | Poultry Science |
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| Main Author: | |
| Format: | Text |
| Language: | English |
| Published: |
Oxford University Press
1967
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| Subjects: | |
| Online Access: | http://ps.oxfordjournals.org/cgi/content/short/46/1/214 https://doi.org/10.3382/ps.0460214 |
| Summary: | THE determination of hematocrit (Hct) is commonly employed as a measure of the erythrocyte concentration in the blood. Most studies in avian hematology have employed macro Hct methods, requiring at least 1 ml. of blood and centrifugation for 30 min. or more. There has, however, been increasing adoption of the more rapid, and more accurate, microcapillary method (McGovern et al., 1955), as developed for mammalian blood from the methods of Guest and Siler (1934), Hamre (1940), Strumia et al. (1954), and others. This method employs glass capillary tubes, 0.06 ml. of blood, and centrifugation for less than 10 min. The micro-Hct method was employed on avian blood in the early 1940’s by Hamre and McHenry (1942) and again by Johnston (1955). Although the method has been used in many avian studies more recently, certain methodological aspects have apparently received little attention. These include the topics of anticoagulation, sampling precision, and . . . |
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