Role of Ca2+ on Triggering Tonoplast Action Potential in Intact Nitella flexilis

Sensitive aequorin was microinjected into the cytoplasm of Nitella flexilis to study the role of Ca2+ in the generation of the tonoplast action potential. The temporal relation between the increase in cytoplasmic Ca2+ and the tonoplast action potential suggested that tonoplast action potential is tr...

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Bibliographic Details
Main Authors: Kikuyama, Munehiro, Shimmen, Teruo
Format: Text
Language:English
Published: Oxford University Press 1997
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Online Access:http://pcp.oxfordjournals.org/cgi/content/short/38/8/941
Description
Summary:Sensitive aequorin was microinjected into the cytoplasm of Nitella flexilis to study the role of Ca2+ in the generation of the tonoplast action potential. The temporal relation between the increase in cytoplasmic Ca2+ and the tonoplast action potential suggested that tonoplast action potential is triggered by an increase in cytoplasmic Ca2+. This is also supported by the fact that Mn2+, extracellularly applied, inhibited both the increase in cytoplasmic Ca2+ and the generation of the tonoplast action potential.