Image contrast of biological materials in high-voltage electron microscopy

A new and simple expression has been formulated for the estimation of contrast of amorphous specimens in the tilted-beam mode of dark-field (DF) imaging in a high-voltage electron microscope. DF contrasts have been calculated for AIDS1.DNA, Salmonella typhimurium DNA,human haemoglobin and myoglobin...

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Published in:Journal of Electron Microscopy
Main Authors: Bhakat, P., Bhattacharya, D.K.
Format: Text
Language:English
Published: Oxford University Press 1997
Subjects:
Physical: Full-length Papers
Sperm whale
Online Access:http://jmicro.oxfordjournals.org/cgi/content/short/46/1/59
https://doi.org/10.1093/oxfordjournals.jmicro.a023490
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spelling fthighwire:oai:open-archive.highwire.org:jmicro:46/1/59 2023-05-15T18:26:41+02:00 Image contrast of biological materials in high-voltage electron microscopy Bhakat, P. Bhattacharya, D.K. 1997-01-01 00:00:00.0 text/html http://jmicro.oxfordjournals.org/cgi/content/short/46/1/59 https://doi.org/10.1093/oxfordjournals.jmicro.a023490 en eng Oxford University Press http://jmicro.oxfordjournals.org/cgi/content/short/46/1/59 http://dx.doi.org/10.1093/oxfordjournals.jmicro.a023490 Copyright (C) 1997, Japanese Society of Microscopy Physical: Full-length Papers TEXT 1997 fthighwire https://doi.org/10.1093/oxfordjournals.jmicro.a023490 2015-02-28T22:28:14Z A new and simple expression has been formulated for the estimation of contrast of amorphous specimens in the tilted-beam mode of dark-field (DF) imaging in a high-voltage electron microscope. DF contrasts have been calculated for AIDS1.DNA, Salmonella typhimurium DNA,human haemoglobin and myoglobin of sperm whale in the range 0.1–3.0 MV and compared with the corresponding bright-field (BF) contrasts. DF contrast is seen to be significantly greaterthan BF contrast for a wide range of microscope apertures (α) and voltages (�). The ratio of DF to BF contrast was observed to increase with increasing � and α for all the aforesaid specimens. Text Sperm whale HighWire Press (Stanford University) Journal of Electron Microscopy 46 1 59 66
institution Open Polar
collection HighWire Press (Stanford University)
op_collection_id fthighwire
language English
topic Physical: Full-length Papers
spellingShingle Physical: Full-length Papers
Bhakat, P.
Bhattacharya, D.K.
Image contrast of biological materials in high-voltage electron microscopy
topic_facet Physical: Full-length Papers
description A new and simple expression has been formulated for the estimation of contrast of amorphous specimens in the tilted-beam mode of dark-field (DF) imaging in a high-voltage electron microscope. DF contrasts have been calculated for AIDS1.DNA, Salmonella typhimurium DNA,human haemoglobin and myoglobin of sperm whale in the range 0.1–3.0 MV and compared with the corresponding bright-field (BF) contrasts. DF contrast is seen to be significantly greaterthan BF contrast for a wide range of microscope apertures (α) and voltages (�). The ratio of DF to BF contrast was observed to increase with increasing � and α for all the aforesaid specimens.
format Text
author Bhakat, P.
Bhattacharya, D.K.
author_facet Bhakat, P.
Bhattacharya, D.K.
author_sort Bhakat, P.
title Image contrast of biological materials in high-voltage electron microscopy
title_short Image contrast of biological materials in high-voltage electron microscopy
title_full Image contrast of biological materials in high-voltage electron microscopy
title_fullStr Image contrast of biological materials in high-voltage electron microscopy
title_full_unstemmed Image contrast of biological materials in high-voltage electron microscopy
title_sort image contrast of biological materials in high-voltage electron microscopy
publisher Oxford University Press
publishDate 1997
url http://jmicro.oxfordjournals.org/cgi/content/short/46/1/59
https://doi.org/10.1093/oxfordjournals.jmicro.a023490
genre Sperm whale
genre_facet Sperm whale
op_relation http://jmicro.oxfordjournals.org/cgi/content/short/46/1/59
http://dx.doi.org/10.1093/oxfordjournals.jmicro.a023490
op_rights Copyright (C) 1997, Japanese Society of Microscopy
op_doi https://doi.org/10.1093/oxfordjournals.jmicro.a023490
container_title Journal of Electron Microscopy
container_volume 46
container_issue 1
container_start_page 59
op_container_end_page 66
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