Image contrast of biological materials in high-voltage electron microscopy

A new and simple expression has been formulated for the estimation of contrast of amorphous specimens in the tilted-beam mode of dark-field (DF) imaging in a high-voltage electron microscope. DF contrasts have been calculated for AIDS1.DNA, Salmonella typhimurium DNA,human haemoglobin and myoglobin...

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Bibliographic Details
Published in:Journal of Electron Microscopy
Main Authors: Bhakat, P., Bhattacharya, D.K.
Format: Text
Language:English
Published: Oxford University Press 1997
Subjects:
Physical: Full-length Papers
Sperm whale
Online Access:http://jmicro.oxfordjournals.org/cgi/content/short/46/1/59
https://doi.org/10.1093/oxfordjournals.jmicro.a023490
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Summary:A new and simple expression has been formulated for the estimation of contrast of amorphous specimens in the tilted-beam mode of dark-field (DF) imaging in a high-voltage electron microscope. DF contrasts have been calculated for AIDS1.DNA, Salmonella typhimurium DNA,human haemoglobin and myoglobin of sperm whale in the range 0.1–3.0 MV and compared with the corresponding bright-field (BF) contrasts. DF contrast is seen to be significantly greaterthan BF contrast for a wide range of microscope apertures (α) and voltages (�). The ratio of DF to BF contrast was observed to increase with increasing � and α for all the aforesaid specimens.

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