Gene expression in haploid embryos of Atlantic salmon
Haploid embryos of Atlantic salmon ( Salmo salar ) were produced by treating eggs with UV-irradiated milt from brook trout ( Salvelinus fontinalis ). Haploidy was confirmed by cytology. Starch gel electrophoresis for 24 biochemical loci indicated that genes were fully activated in haploids—the degre...
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| Format: | Text |
| Language: | English |
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Oxford University Press
1983
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| Online Access: | http://jhered.oxfordjournals.org/cgi/content/short/74/1/19 |
| Summary: | Haploid embryos of Atlantic salmon ( Salmo salar ) were produced by treating eggs with UV-irradiated milt from brook trout ( Salvelinus fontinalis ). Haploidy was confirmed by cytology. Starch gel electrophoresis for 24 biochemical loci indicated that genes were fully activated in haploids—the degree of staining was similar to that of diploids. Dimer and tetramer assembly of protein products from multiple loci was the same in haploids and diploids. The genetic interpretation of Isozyme patterns was more straightforward in haploids than in diplolds. Fixation of alternate alleles of duplicate loci was demonstrated for IDH -( 3,4 ) and LDH -( 3,4 ). |
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