A simple genetic identification method for Northeast Atlantic smoothhound sharks (Mustelus spp.)

<qd> Farrell, E. D., Clarke, M. W., and Mariani, S. 2009. A simple genetic identification method for Northeast Atlantic smoothhound sharks ( Mustelus spp.). – ICES Journal of Marine Science, 66: 561–565. </qd>Considerable ambiguity exists in the identification of the commercially valuabl...

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Bibliographic Details
Published in:ICES Journal of Marine Science
Main Authors: Farrell, Edward D., Clarke, Maurice W., Mariani, Stefano
Format: Text
Language:English
Published: Oxford University Press 2009
Subjects:
Online Access:http://icesjms.oxfordjournals.org/cgi/content/short/66/3/561
https://doi.org/10.1093/icesjms/fsn218
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Summary:<qd> Farrell, E. D., Clarke, M. W., and Mariani, S. 2009. A simple genetic identification method for Northeast Atlantic smoothhound sharks ( Mustelus spp.). – ICES Journal of Marine Science, 66: 561–565. </qd>Considerable ambiguity exists in the identification of the commercially valuable smoothhound sharks ( Mustelus spp.) in the Northeast (NE) Atlantic. The lack of a clear and accurate method of identification prevents the collation of reliable species-specific landings and survey data for these fish and hinders the accurate delineation of the distribution ranges of species and stock boundaries, making it impossible to apply sound species-specific conservation and management strategies. This paper reports on the development of a multiplex PCR reaction that utilizes a set of mtDNA primers for the identification of Mustelus asterias , Mustelus mustelus , and Galeorhinus galeus . The high throughput method allows for the rapid and cost-effective identification of large numbers of samples; its application to 431 fish collected between 2006 and 2008 also raises important questions regarding the biogeography of the genus Mustelus in the NE Atlantic.