A method for preparing large numbers of otolith sections for viewing by scanning electron microscope

In an investigation of the age structure of a population of Notothenia coriiceps Richardson at the South Orkney Islands, Antarctica, the microstructure of otoliths was examined. Progress is reported on a method used for processing large numbers of otoliths using a scanning electron microscope (SEM)....

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Bibliographic Details
Published in:ICES Journal of Marine Science
Main Authors: Ashford, J. R., Robinson, K., White, M. G.
Format: Text
Language:English
Published: Oxford University Press 1993
Subjects:
Online Access:http://icesjms.oxfordjournals.org/cgi/content/short/50/2/227
https://doi.org/10.1006/jmsc.1993.1024
Description
Summary:In an investigation of the age structure of a population of Notothenia coriiceps Richardson at the South Orkney Islands, Antarctica, the microstructure of otoliths was examined. Progress is reported on a method used for processing large numbers of otoliths using a scanning electron microscope (SEM). Compared with previous SEM techniques, which normally prepare otoliths individually for viewing, this allowed larger sample sizes to be examined. Compared with similar light microscope techniques, this method gave enhanced resolution, particularly for discerning edge structure, and thus may facilitate present methods of ageing fish populations, especially for fishery work in the Antarctic where otoliths are often small and difficult to interpret using conventional techniques.