A 'capillary racetrack' method for isolation of magnetotactic bacteria

A capillary tube was developed in which an inoculum of magnetotactic bacteria that contained only a few contaminants could be separated from crude sediment in a few minutes. Sterile fluid was placed on one side of a wetted cotton plug and sediment was placed on the other side. Magnetotactic bacteria...

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Bibliographic Details
Published in:FEMS Microbiology Letters
Main Authors: Wolfe, R.S., Thauer, R.K., Pfennig, N.
Format: Text
Language:English
Published: Oxford University Press 1987
Subjects:
Online Access:http://femsec.oxfordjournals.org/cgi/content/short/3/1/31
https://doi.org/10.1111/j.1574-6968.1987.tb02335.x
Description
Summary:A capillary tube was developed in which an inoculum of magnetotactic bacteria that contained only a few contaminants could be separated from crude sediment in a few minutes. Sterile fluid was placed on one side of a wetted cotton plug and sediment was placed on the other side. Magnetotactic bacteria migrated quickly through the cotton toward the south pole of a stirring-bar magnet placed at the closed end of the capillary. Protozoa and chemotactic bacteria were significantly delayed in passage through the cotton.