Εποχικές, βιοχημικές και φυσιολογικές αποκρίσεις της τσιπούρας ( Sparus aurata ): συσχέτιση με τη θερμοκρασία της θάλασσας και την κλιματική αλλαγή

• Main Authors: Feidantsis, Konstantinos, Φειδάντσης, Κωνσταντίνος
• Format: Doctoral or Postdoctoral Thesis
• Language: Greek
• Published: Aristotle University Of Thessaloniki (AUTH) 2012
• Subjects: Τσιπούρα ( Sparus aurata); Μοριακές; μεταβολικές και αντιοξειδωτικές αποκρίσεις; Θερμοκρασία θάλασσας; Υδατοκαλλιέργειες; Gilthead sea bream (Sparus aurata); Molecular; metabolic and antioxidant responses; Sea temperature; Aquaculture; Φυσικές Επιστήμες; Βιολογία; Natural Sciences; Biological Sciences; Ocean acidification
• Online Access: http://hdl.handle.net/10442/hedi/34216; https://doi.org/10.12681/eadd/34216

Increased atmospheric levels of CO2 due to human activities and the subsequent Climate Change with effects summarizing except of the world temperature increase, to the ocean acidification, the reduction of the dissolved oxygen in marine ecosystems and the increase of the sea level, trigger a series of physical and chemical alterations that affect all levels of biological organization. Therefore, temperature is an environmental factor of great importance, since it significantly affects organisms’ activity as well as growth and developmental rates. Fish is the main group of aquatic organisms, and as a diet it is one of the main protein sources, in many countries, including Greece. The aim of the present study was to determine the biochemical and metabolic responses of one well studied and commercial species, the gilthead sea bream (Sparus aurata), in different water temperatures with or without the presence of CO2, and the seasonal pattern of these responses. Specifically, in several tissues of the particular species, we determined the induced levels of heat shock proteins Hsp70 and Hsp90, and the phosphorylated levels p38 MAPK, p54/46 JNK, p44/42 MAPK (ERKs), as well as expression levels of the transcriptional factor Hydroxy - Hifα and Ubiquitin. Cell metabolism adjustments were assessed by determining the activity of enzymes such as lactate dehydrogenase (L-LDH), citrate synthetase (CS) and β-hydroxyacyl CoA dehydrogenase (HOAD). Additionally we studied the kinetics of the above mentioned enzymes and antioxidant enzymes such as xanthine oxidase (XO), catalase (CAT), glutathione peroxidise (GPx) and superoxide dismoutase (SOD), as well as the determination of thiobarbituric acid reactive substances (TBARs). Levels of glucose, triglycerides and lactate were determined in blood plasma. A few reports have shown a direct relationship between phosphorylation of p38 MAPK and induction of Hsp70. This evidence is derived only from studies on mammalian tissues. A particular experimental procedure species has also shown ...