Μοριακή ποικιλότητα και αφθονία προκαρυωτικών οργανισμών του πεπτικού συστήματος της καραβίδας ( nephrops norvegicus) σε φυσικούς και εκτρεφόμενο.

• Main Authors: Μεζίτη, Αλεξάνδρα, Meziti, Alexandra
• Format: Doctoral or Postdoctoral Thesis
• Language: Greek
• Published: University of Thessaly (UTH) 2012
• Subjects: Nephrops norvegius; Μεσέντερο; Βακτήρια; Γονίδια; 16S rRNA; Εκτροφές; Photobacterium; Προβιοτικά; Bacteria; Midgut; Rearing; Probiotics; Γεωπονικές Επιστήμες και Κτηνιατρική; Άλλες Γεωπονικές Επιστήμες; Agricultural and Veterinary Sciences; Other Agricultural Sciences; Norway; Northeast Atlantic
• Online Access: http://hdl.handle.net/10442/hedi/26764; https://doi.org/10.12681/eadd/26764

2. The Norway lobster Nephrops norvegicus, also known as Dublin bay prawn is a Decapod Crustacean of the Family Nephropidae that is widely distributed in silty areas, of the continental shelf and the upper continental slope, of the Northeast Atlantic and the Mediterranean Sea. The Norway lobster is a highly commercial species in the areas where it is found. Efforts for the commercial rearing of the species have not succeeded yet due to lack of knowledge on the nutritional habits of the species on rearing conditions and on its exact nutritional demands. 3. This thesis studied for the first time the gut bacterial diversity of Nephrops norvegicus. Studies were performed in natural and reared populations, where different food sources were provided. The project targeted in finding the factors that shape gut bacterial diversity and in detecting symbiotic, non toxic and non pathogenic bacterial species that can influence the digestive functions of N. norvegicus and could potentially be used as probiotics in future rearing efforts. 4. For the study of the natural populations N. norvegicus individuals were collected from Pagasitikos gulf during different months in 2007. The digestive tract was extracted and evacuated, followed by DNA extraction. DNA was analyzed with the use of molecular tools studying 16S rRNA and the Intergenic Transcribed Spacer (ITS). The 16S rRNA diversity was studied with the use of clone libraries and Fluorescence in Situ Hybridization (FISH) while the ITS diversity was studied with the use of clone libraries and Automated Ribosomal Intergenic Spacer Analysis (ARISA). The study of the ITS diversity with the use of ARISA showed that gut bacterial microdiversity was influenced by sampling season while other factors that were checked such as sex or weight had no influence. Seasonal differences in Pagasitikos gulf, and mostly in the areas where N. norvegicus is found, are based in changes in food availability and quality and not in physicochemical factors. In this area temperature and salinity are ...