Cortisol Regulates Eel ( Anguilla anguilla ) Aquaporin 3 (AQP3) mRNA Expression Levels in Gill

Previous studies in eel (Anguilla anguilla) gill have shown that the expression of the aquaporin 3 (AQP3) water and small solute channel is dramatically decreased (mRNA abundance decreased by up to 97%) when these euryhaline fish are acclimated from freshwater (FW) to seawater (SW). However, AQP3 mR...

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Bibliographic Details
Published in:General and Comparative Endocrinology
Main Authors: Cutler, Christopher P., Phillips, Clair, Hazon, Neil, Cramb, Gordon
Format: Text
Language:unknown
Published: Digital Commons@Georgia Southern 2007
Subjects:
Cortisol
Eel
Anguilla anguilla
Aquaporin 3
AQP3
mRNA
Expression levels
Gill
Biochemistry
Biophysics
and Structural Biology
Biology
Online Access:https://digitalcommons.georgiasouthern.edu/biology-facpubs/56
https://doi.org/10.1016/j.ygcen.2007.01.031
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Summary:Previous studies in eel (Anguilla anguilla) gill have shown that the expression of the aquaporin 3 (AQP3) water and small solute channel is dramatically decreased (mRNA abundance decreased by up to 97%) when these euryhaline fish are acclimated from freshwater (FW) to seawater (SW). However, AQP3 mRNA expression levels in the intestine following SW-acclimation do not change. The SW-acclimating corticosteroid hormone, cortisol has previously been shown to regulate the expression of aquaporins (particularly AQP1) in eel osmoregulatory tissues in a tissue-specific and isoform-specific fashion. AQP1 is up-regulated in intestine and oesophagus, but down-regulated in kidney, following SW-acclimation in these fish. This study extends knowledge of the regulation of aquaporin expression by cortisol in the eel and shows that elevated levels of this hormone down-regulate AQP3 mRNA expression in the gill in a similar manner to SW-acclimation. However, the smaller magnitude of the changes in branchial AQP3 expression induced by cortisol-infusion (around a 60% decrease), in comparison to those occurring following SW-acclimation, suggest that other factors must also contribute to AQP3 down-regulation. In a similar fashion to the regulation of AQP1 by cortisol, changes in AQP3 expression following hormone infusion appear to be tissue-specific, as little effect was seen on the level of AQP3 expression in the intestine. Again the apparent lack of change in intestinal AQP3 expression following cortisol-infusion mimicked the invariant level of intestinal AQP3 mRNA abundance following SW-acclimation.

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