Image_4_Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease.PNG

Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-ass...

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Bibliographic Details
Main Authors: Johanna Marsian, Daniel L. Hurdiss, Neil A. Ranson, Anneli Ritala, Richard Paley, Irene Cano, George P. Lomonossoff
Format: Still Image
Language:unknown
Published: 2019
Subjects:
Botany
Plant Biology
Plant Systematics and Taxonomy
Plant Cell and Molecular Biology
Plant Developmental and Reproductive Biology
Plant Pathology
Plant Physiology
Plant Biology not elsewhere classified
Atlantic cod nervous necrosis virus
virus-like particles
transient expression
sea bass
protective immunity
atlantic cod
Online Access:https://doi.org/10.3389/fpls.2019.00880.s004
https://figshare.com/articles/Image_4_Plant-Made_Nervous_Necrosis_Virus-Like_Particles_Protect_Fish_Against_Disease_PNG/8831207
Description
Summary:Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

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