Data_Sheet_1_Bioprospecting of inhibitors of EPEC virulence from metabolites of marine actinobacteria from the Arctic Sea.docx

A considerable number of antibacterial agents are derived from bacterial metabolites. Similarly, numerous known compounds that impede bacterial virulence stem from bacterial metabolites. Enteropathogenic Escherichia coli (EPEC) is a notable human pathogen causing intestinal infections, particularly...

Full description

Bibliographic Details
Main Authors: Tuomas Pylkkö, Yannik Karl-Heinz Schneider, Teppo Rämä, Jeanette Hammer Andersen, Päivi Tammela
Format: Dataset
Language:unknown
Published: 2024
Subjects:
Microbiology
Microbial Genetics
Microbial Ecology
Mycology
antivirulence
EPEC
arctic marine microorganisms
bioprospecting
actinobacteria
Arctic
Online Access:https://doi.org/10.3389/fmicb.2024.1432475.s001
https://figshare.com/articles/dataset/Data_Sheet_1_Bioprospecting_of_inhibitors_of_EPEC_virulence_from_metabolites_of_marine_actinobacteria_from_the_Arctic_Sea_docx/26877931
Description
Summary:A considerable number of antibacterial agents are derived from bacterial metabolites. Similarly, numerous known compounds that impede bacterial virulence stem from bacterial metabolites. Enteropathogenic Escherichia coli (EPEC) is a notable human pathogen causing intestinal infections, particularly affecting infant mortality in developing regions. These infections are characterized by microvilli effacement and intestinal epithelial lesions linked with aberrant actin polymerization. This study aimed to identify potential antivirulence compounds for EPEC infections among bacterial metabolites harvested from marine actinobacteria (Kocuria sp. and Rhodococcus spp.) from the Arctic Sea by the application of virulence-based screening assays. Moreover, we demonstrate the suitability of these antivirulence assays to screen actinobacteria extract fractions for the bioassay-guided identification of metabolites. We discovered a compound in the fifth fraction of a Kocuria strain that interferes with EPEC-induced actin polymerization without affecting growth. Furthermore, a growth-inhibiting compound was identified in the fifth fraction of a Rhodococcus strain. Our findings include the bioassay-guided identification, HPLC-MS-based dereplication, and isolation of a large phospholipid and a likely antimicrobial peptide, demonstrating the usefulness of this approach in screening for compounds capable of inhibiting EPEC virulence.

Similar Items