Table_1_Identification of two novel papillomaviruses in belugas.xlsx

Introduction Papillomaviruses (PVs) can cause hyperplasia in the skin and mucous membranes of humans, mammals, and non-mammalian animals, and are a significant risk factor for cervical and genital cancers. Methods Using next-generation sequencing (NGS), we identified two novel strains of papillomavi...

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Bibliographic Details
Main Authors: Youyou Li, Meifang Xiao, Yun Zhang, Zihan Li, Shijie Bai, Haoxiang Su, Ruoyan Peng, Gaoyu Wang, Xiaoyuan Hu, Xinran Song, Xin Li, Chuanning Tang, Gang Lu, Feifei Yin, Peijun Zhang, Jiang Du
Format: Dataset
Language:unknown
Published: 2023
Subjects:
Microbiology
Microbial Genetics
Microbial Ecology
Mycology
diversity
novel PVs
evolution
beluga whale
next-generation sequencing
Beluga
Beluga whale
Beluga*
Delphinapterus leucas
Killer Whale
Online Access:https://doi.org/10.3389/fmicb.2023.1165839.s001
https://figshare.com/articles/dataset/Table_1_Identification_of_two_novel_papillomaviruses_in_belugas_xlsx/23781315
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Summary:Introduction Papillomaviruses (PVs) can cause hyperplasia in the skin and mucous membranes of humans, mammals, and non-mammalian animals, and are a significant risk factor for cervical and genital cancers. Methods Using next-generation sequencing (NGS), we identified two novel strains of papillomavirus, PV-HMU-1 and PV-HMU-2, in swabs taken from belugas (Delphinapterus leucas) at Polar Ocean Parks in Qingdao and Dalian. Results We amplified the complete genomes of both strains and screened ten belugas and one false killer whale (Pseudorca crassidens) for the late gene (L1) to determine the infection rate. In Qingdao, 50% of the two sampled belugas were infected with PV-HMU-1, while the false killer whale was negative. In Dalian, 71% of the eight sampled belugas were infected with PV-HMU-2. In their L1 genes, PV-HMU-1 and PV-HMU-2 showed 64.99 and 68.12% amino acid identity, respectively, with other members of Papillomaviridae. Phylogenetic analysis of combinatorial amino acid sequences revealed that PV-HMU-1 and PV-HMU-2 clustered with other known dolphin PVs but formed distinct branches. PVs carried by belugas were proposed as novel species under Firstpapillomavirinae. Conclusion The discovery of these two novel PVs enhances our understanding of the genetic diversity of papillomaviruses and their impact on the beluga population.

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