Image_2_Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.tif

• Main Authors: Rasaq A. Ojasanya, Ian A. Gardner, David Groman, Sonja Saksida, Matthew E. Saab, Krishna K. Thakur
• Format: Still Image
• Language: unknown
• Published: 2022
• Subjects: Animal Systematics and Taxonomy; Animal Physiology - Biophysics; Animal Physiology - Cell; Animal Physiology - Systems; Animal Behaviour; Animal Cell and Molecular Biology; Animal Developmental and Reproductive Biology; Animal Immunology; Animal Neurobiology; Animal Physiological Ecology; Animal Structure and Function; Veterinary Anaesthesiology and Intensive Care; Veterinary Anatomy and Physiology; Veterinary Diagnosis and Diagnostics; Veterinary Epidemiology; Veterinary Immunology; Veterinary Medicine; Veterinary Microbiology (excl. Virology); Veterinary Parasitology; Veterinary Pathology; Veterinary Pharmacology; Veterinary Surgery; Veterinary Virology; Veterinary Sciences not elsewhere classified; MALDI-TOF MS; main spectral profile; Yersinia ruckeri; aquaculture; validation; atlantic cod; Atlantic salmon; Gadus morhua; Salmo salar
• Online Access: https://doi.org/10.3389/fvets.2022.1031373.s002; https://figshare.com/articles/figure/Image_2_Development_and_validation_of_main_spectral_profile_for_rapid_identification_of_Yersinia_ruckeri_isolated_from_Atlantic_salmon_using_matrix-assisted_laser_desorption_ionization_time-of-flight_mass_spectrometry_tif/21366708

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms. The accuracy of bacterial identification using MALDI-TOF MS depends on main spectral profiles (MSPs) provided in a quality-assured commercial reference library, which requires ongoing improvement. This study aimed to develop and validate an in-house MALDI-TOF MS MSP to rapidly identify Yersinia ruckeri isolated from Atlantic salmon (Salmo salar). The novel MSP was prepared using an isolate of Y. ruckeri recovered from Atlantic salmon and confirmed by 16S rRNA gene sequencing. Subsequently, a validation set which comprises 29 isolates of Y. ruckeri were examined from three fishes: Atlantic salmon (Salmo salar) (n = 26), American eel (Anguilla rostrata) (n = 1), and Atlantic cod (Gadus morhua) (n = 2). These isolates were randomly selected from the Atlantic Veterinary College, Aquatic Diagnostic Services Bacteriology Laboratory's culture collection to validate the novel MSP. Analytical sensitivity of MALDI-TOF MS using the novel MSP to identify the validation set was 86.2%. Repeatability was assessed by acquiring spectra from 30 different spots of a randomly-selected isolate of Y. ruckeri, and analyzed spectra from each spot were compared against the novel MSP. The coefficient of variation was 3.3%. The novel MSP clustered with Bruker MSPs (n = 3) of Y. ruckeri in the reference library and did not falsely identify any closely related bacteria to Y. ruckeri. This study reports the development of a novel MSP of high analytical sensitivity and specificity for rapid identification of Y. ruckeri using MALDI-TOF MS.