Data_Sheet_1_A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats.PDF

• Main Authors: Hui Chen, Yu Chen, Huizhen Zheng, Xingwei Xiang, Lu Xu
• Format: Dataset
• Language: unknown
• Published: 2022
• Subjects: Clinical and Sports Nutrition; Dietetics and Nutrigenomics; Nutritional Physiology; Public Nutrition Intervention; Nutrition and Dietetics not elsewhere classified; Food Chemistry and Molecular Gastronomy (excl. Wine); Food Nutritional Balance; Animal Nutrition; Crop and Pasture Nutrition; angiotensin-I-converting enzyme (ACE); oyster peptides; hypotensive; molecular docking; simulated gastro-intestinal digestion; Crassostrea gigas
• Online Access: https://doi.org/10.3389/fnut.2022.981163.s001; https://figshare.com/articles/dataset/Data_Sheet_1_A_novel_angiotensin-I-converting_enzyme_inhibitory_peptide_from_oyster_Simulated_gastro-intestinal_digestion_molecular_docking_inhibition_kinetics_and_antihypertensive_effects_in_rats_PDF/20548383

In this study, a novel peptide, AEYLCEAC with high angiotensin-I-converting enzyme inhibitory (ACEI) activity was screened from oyster (Crassostrea gigas) hydrolysates, which was obtained from simulated gastro-intestinal digestion. Candidate peptides were confirmed to have a higher binding to angiotensin-I-converting enzyme (ACE) than the positive drug phosphoinic tripeptide calculated by Discovery Studio, and AEYLCEAC showed the highest ACE inhibition rate in vitro with a IC 50 of 4.287 mM. Lineweaver-Burk plots confirmed that the peptidic inhibitory type of ACE is competitive. The molecular docking showed that ACEI activity of the AEYLCEAC was mainly due to the hydrogen bonding interactions with the active pockets (S1 and S2) of ACE. In vivo, AEYLCEAC effectively reduced diastolic blood pressure (DBP) and Systolic blood pressure (SBP) in hypertensive rats. These results indicate that AEYLCEAC might act as a helpful ingredient in functional foods or pharmaceuticals for the prevention and treatment of hypertension.