Characterization of protein-encodingcDNA clones from Stellaria longipes (Caryophyllaceae): Gene expression and evolution.

• Main Authors: Zhang, Xing-Hai., University of Calgary (Canada).
• Format: Text
• Language: English
• Subjects: Biology; Molecular.; Botany.; Canada; Goldie; Stellaria longipes
• Online Access: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:NN94017; http://ezproxy.fau.edu; http://digitool.fcla.edu:80/R/?func=dbin-jump-full&object_id=40279

A cDNA library was constructed from a genotype of Stellaria longipes Goldie (family Caryophyllaceae). Five clones encoding (1) mitochondrial cytochrome c, (2) triose phosphate isomerase (TPI), (3) the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS), (4) a dehydrin-like protein, and (5) S-adenosyl- scL-methionine:trans-caffeoyl-CoA 3-O-methyltransferase (CCoAMT) have been isolated, sequenced and analyzed. These cDNAs were used to investigate the gene complexity and expression in genotypes of S. longipes. Southern blot analysis showed that the S. longipes genome contained multiple homologous sequences for cytochrome c, rbcS, TPI, and CCoAMT. The CCoAMT cDNA-corresponding gene did not contain an intron in its coding sequences. Certain degrees of nucleotide and amino acid sequence variations in the CCoAMT gene were found between S. longipes genotypes from different habitats. There was a certain degree of tissue specificity in gene expression for cytochrome c, TPI and CCoAMT. Genotypic variation in total TPI activity was observed. Expression of the rbcS gene was responsive to growth conditions with more rbcS mRNA at warm temperature and long-day photoperiod. Dehydrin gene transcription was induced by treatment of abscisic acid, polyethelene glycol, or drought, but not by flooding. Phylogenetic analysis or the S. longipes cytochrome c sequence revealed an odd grouping of S. longipes and Arabidopsis thaliana with fungi and separation from other plants. Gene paralogy was proposed to be the major factor contributing to the dissimilarity of cytochrome c sequences between S. longipes and other plants. Comparative study of the TPI amino acid sequences indicated that the TPI from S. longipes was more closely related to cytosolic TPIs from other plants than it was to TPIs from prokaryotes, and supported the nuclear origin for both cytosolic and plastidic TPI genes. Analysis of the rbcS amino acid and nucleotide sequences showed that among 10 higher plant families including 4 monocot, 14 dicot and 1 gymnosperm taxa tested, the rbcS of S. longipes, along with Silene pratensis (Caryophyllaceae), was more closely related to the families Solanaceae, Brassicaceae and Fabaceae than it was to other families. Thesis (Ph.D.)--University of Calgary (Canada), 1994. A cDNA library was constructed from a genotype of Stellaria longipes Goldie (family Caryophyllaceae). Five clones encoding (1) mitochondrial cytochrome c, (2) triose phosphate isomerase (TPI), (3) the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS), (4) a dehydrin-like protein, and (5) S-adenosyl- scL-methionine:trans-caffeoyl-CoA 3-O-methyltransferase (CCoAMT) have been isolated, sequenced and analyzed. These cDNAs were used to investigate the gene complexity and expression in genotypes of S. longipes. Southern blot analysis showed that the S. longipes genome contained multiple homologous sequences for cytochrome c, rbcS, TPI, and CCoAMT. The CCoAMT cDNA-corresponding gene did not contain an intron in its coding sequences. Certain degrees of nucleotide and amino acid sequence variations in the CCoAMT gene were found between S. longipes genotypes from different habitats. There was a certain degree of tissue specificity in gene expression for cytochrome c, TPI and CCoAMT. Genotypic variation in total TPI activity was observed. Expression of the rbcS gene was responsive to growth conditions with more rbcS mRNA at warm temperature and long-day photoperiod. Dehydrin gene transcription was induced by treatment of abscisic acid, polyethelene glycol, or drought, but not by flooding. Phylogenetic analysis or the S. longipes cytochrome c sequence revealed an odd grouping of S. longipes and Arabidopsis thaliana with fungi and separation from other plants. Gene paralogy was proposed to be the major factor contributing to the dissimilarity of cytochrome c sequences between S. longipes and other plants. Comparative study of the TPI amino acid sequences indicated that the TPI from S. longipes was more closely related to cytosolic TPIs from other plants than it was to TPIs from prokaryotes, and supported the nuclear origin for both cytosolic and plastidic TPI genes. Analysis of the rbcS amino acid and nucleotide sequences showed that among 10 higher plant families including 4 monocot, 14 dicot and 1 gymnosperm taxa tested, the rbcS of S. longipes, along with Silene pratensis (Caryophyllaceae), was more closely related to the families Solanaceae, Brassicaceae and Fabaceae than it was to other families. School code: 0026. hdl